Behavioral observation of Xenopus tadpole swimming for neuroscience labs.
Behavioral observation of Xenopus tadpole swimming for neuroscience labs.
复制标题
神经科学实验室爪蟾蝌蚪游泳的行为观察。
DOI：
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
Nicola J Porter
中科院分区：
文献类型：
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作者：
Wen;M. Wagner;Nicola J Porter
神经科学实验室受益于经过充分研究的神经通路介导的可靠、易于监控的神经反应。非洲爪蟾蝌蚪已被用作运动控制研究中的简单脊椎动物模型制备。蝌蚪游泳行为不同方面的大多数神经元通路已经被揭示。其中包括皮肤机械感觉触觉和松果体眼感光通路，其激活可以启动游泳，以及负责停止游泳的水泥腺压力传感通路。后脑的简单横断可以切断松果体眼和水泥腺通路与脊髓游泳回路的连接，导致相应功能的丧失。此外，一些针对神经传递的药理学实验可以设计来影响游泳，并且荧光缀合的 α-银环蛇毒素可用于标记神经肌肉接头处的烟碱受体。这些实验可以很容易地适用于本科生神经科学教学实验室。还讨论了一些实验可能扩展到更复杂的药理学或神经生理学实验室的可能性。
Neuroscience labs benefit from reliable, easily-monitored neural responses mediated by well-studied neural pathways. Xenopus laevis tadpoles have been used as a simple vertebrate model preparation in motor control studies. Most of the neuronal pathways underlying different aspects of tadpole swimming behavior have been revealed. These include the skin mechanosensory touch and pineal eye light-sensing pathways whose activation can initiate swimming, and the cement gland pressure-sensing pathway responsible for stopping swimming. A simple transection in the hindbrain can cut off the pineal eye and cement gland pathways from the swimming circuit in the spinal cord, resulting in losses of corresponding functions. Additionally, some pharmacological experiments targeting neurotransmission can be designed to affect swimming and, fluorescence-conjugated α-bungarotoxin can be used to label nicotinic receptors at neuromuscular junctions. These experiments can be readily adapted for undergraduate neuroscience teaching labs. Possible expansions of some experiments for more sophisticated pharmacological or neurophysiological labs are also discussed.
模块化实验室练习，用于分析斑马鱼运动行为的发展。
影响因子：
2
作者：
McKeown,KellyAnne;Downes,GeraldB;Hutson,LaraD
通讯作者：
Hutson,LaraD