Polymerized human placenta haemoglobin attenuates myocardial injury and aortic endothelial dysfunction in a rat model of severe burns
Polymerized human placenta haemoglobin attenuates myocardial injury and aortic endothelial dysfunction in a rat model of severe burns
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DOI：
10.1080/21691401.2017.1396999
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发表时间：
2018-08
期刊：
影响因子：
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通讯作者：
Zhenyu Zhang;Yingyi Zhang;Yan Deng;Shen Li-;Wentao Zhou;Chengmin Yang;Xuewen Xu;Tao Li
中科院分区：
文献类型：
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作者：
Zhenyu Zhang;Yingyi Zhang;Yan Deng;Shen Li-;Wentao Zhou;Chengmin Yang;Xuewen Xu;Tao Li
本研究旨在探讨聚合人胎盘血红蛋白（PolyPHb）对严重烧伤后心功能不全的影响。将60只雄性SD大鼠随机分为假手术组、烧伤组和烧伤+ PolyPHb组。采用大鼠30%体表面积三度烧伤模型，测定大鼠血流动力学、心肌酶释放及主动脉内皮细胞超微结构和功能。与烧伤组相比，PolyPHb（0.5 gHb/kg）显著改善了平均动脉压、左心室发展压（LVDP）、最大LVDP升高和降低速率，并降低了左心室舒张末期压。Burn + PolyPHb组的心肌酶包括CK-MB和肌钙蛋白I的血浆水平也显著下调。此外，PolyPHb治疗显著恢复了严重烧伤和血管内皮病理改变所损害的内皮依赖性舒张功能。因此，我们的数据表明，PolyPHb可以限制严重烧伤引起的心肌损伤，这与保护主动脉内皮有关。
This study was designed to investigate the effect of polymerized human placenta haemoglobin (PolyPHb) on cardiac dysfunction after severe burns. A total of 60 male Sprague-Dawley rats were randomly divided into 3 groups: Sham, Burn and Burn + PolyPHb groups. Rats were subjected to third-degree burns to 30% of total body surface area and the haemodynamics, cardiac enzyme release and aortic endothelium ultrastructure/function were measured. PolyPHb (0.5 gHb/kg) greatly improved mean arterial pressure, left ventricular developed pressure (LVDP), maximum LVDP increase and decrease rate and reduced left ventricular end-diastolic pressure as compared to the Burn group. The plasma levels of cardiac enzyme including CK-MB and troponin I were also significantly down-regulated in the Burn + PolyPHb group. In addition, PolyPHb treatment markedly restored the endothelium-dependent relaxation impaired by severe burns and pathological changes of endothelium in aorta. Therefore, our data suggest that PolyPHb can limit severe burn-induced myocardial injury, which is associated with protection of aortic endothelium.