MOLECULAR-CLONING OF MOUSE PLACENTAL-LACTOGEN CDNA
MOLECULAR-CLONING OF MOUSE PLACENTAL-LACTOGEN CDNA
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DOI：
10.1073/pnas.83.22.8496
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发表时间：
1986-11-01
影响因子：
11.1
通讯作者：
LINZER, DIH
中科院分区：
文献类型：
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作者：
JACKSON, LL;COLOSI, P;LINZER, DIH
我们从噬菌体中分离出23kda的小鼠胎盘乳原II (mPL-II) cDNA克隆。含BALB/c胎盘RNA合成cDNA的gt11表达文库。通过杂交选择的胎盘mRNA到mPL-II cDNA克隆的体外翻译产生26kda的多肽，其大小与预期的前体蛋白相同，并通过抗mPL-II抗血清免疫沉淀。mPL-II cDNA克隆杂交到一个1.0千碱基的胎盘特异性mRNA。这种mRNA在胎盘的胎儿部分中发现，早在妊娠第10天就出现，并在第12天增加到最大水平。确定了mPL-II cDNA的核苷酸序列。该序列包含一个开放阅读框，编码222个氨基酸的多肽，其中氨基末端的31个氨基酸构成分泌信号序列。预测的分泌蛋白与小鼠泌乳素氨基酸同源性为51%。
We have isolated a cDNA clone for the 23-kDa mouse placental lactogen II (mPL-II) from a phage .lambda.gt11 expression library containing cDNA synthesized from BALB/c placental RNA. Translation in vitro of placental mRNA selected by hybridization to the mPL-II cDNA clones yields a 26-kDa polypeptide that is the size of the expected precursor protein and that is immunoprecipitated with anti-mPL-II antiserum. The mPL-II cDNA clones hybridize to a 1.0-kilobase placental-specific mRNA. This mRNA, found in the fetal portion of the placenta, appears as early as day 10 of gestation and increases to a maximal level by day 12. The mPL-II cDNA nucleotide sequence has been determined. This sequence contains an open reading frame encoding a polypeptide of 222 amino acids with the amino-terminal 31 amino acids forming the signal sequence for secretion. The predicted secreted protein has 51% amino acid homology with mouse prolactin.