Four-dimensional visualization of zebrafish cardiovascular and vessel dynamics by a structured illumination microscope with electrically tunable lens
Four-dimensional visualization of zebrafish cardiovascular and vessel dynamics by a structured illumination microscope with electrically tunable lens
复制标题
通过带有电动可调镜头的结构照明显微镜对斑马鱼心血管和血管动力学进行四维可视化
DOI：
10.1364/boe.382114
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发表时间：
2020-02-01
影响因子：
3.4
通讯作者：
Li Hui
中科院分区：
文献类型：
--
作者：
Chen Chong;Li Simin;Li Hui
我们建立了一个四维（4D）显微镜方法，使用结构照明的光学轴向成像与电调谐透镜。凭借其快速成像能力，斑马鱼和脑血管的心血管系统的动态成像的基础上，每秒两个堆栈（25层）的横向/轴向分辨率分别为0.6 μ m和1.8 μ m的覆盖范围。时间推移成像清楚地显示了跳动的心脏在不同的心脏相位和不同区域的血管的不同流动性的收缩-舒张反应。这种新的4D技术将有助于在小型动物中对器官功能，生成以及药物反应进行体内成像。(C)2020年美国光学学会根据OSA开放获取出版协议的条款
We established a four-dimensional (4D) microscopy method using structured illumination for optical axial imaging with an electrically tunable lens. With its fast imaging capability, the dynamics of the cardiovascular system of the zebrafish and cerebral vessels were imaged based on the coverage of two stacks (25 layers) per second with lateral /axial resolutions of 0.6 mu m and 1.8 mu m, respectively. Time lapse imaging clearly shows the contractile-relaxation response of the beating heart at different cardiac phases and with different mobilities of blood vessels in different regions. This new 4D technique will facilitate in vivo imaging of organ function, generation, as well as drug responses in small-sized animals. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement