Mechanisms of tomato mosaic virus RNA replication and its inhibition by the host resistance factor Tm-1
Mechanisms of tomato mosaic virus RNA replication and its inhibition by the host resistance factor Tm-1
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DOI：
10.1016/j.coviro.2014.08.005
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发表时间：
2014-12-01
影响因子：
5.9
通讯作者：
Ishikawa, Masayuki
中科院分区：
文献类型：
--
作者：
Ishibashi, Kazuhiro;Ishikawa, Masayuki
在植物免疫系统中，由优势抗性基因编码的传感蛋白在病原体感染时激活防御反应。番茄花叶病毒（ToMV）抗性基因Tm-1是特殊的，因为它可以抑制ToMV的增殖而不引起防御反应。几条线索的证据表明，Tm-1编码直接抑制ToMV RNA复制。Tm-1基因产物是通过使用ToMV RNA的无细胞翻译和复制系统纯化抑制蛋白来鉴定的。利用该系统进一步分析表明，Tm-1结合的ToMV复制蛋白保留了结合膜的能力，而Tm-1抑制了膜上复制复合体的形成。
In the plant immune system, sensor proteins encoded by dominant resistance genes activate a defense response upon pathogen infection. The tomato mosaic virus (ToMV) resistance gene Tm-1 is exceptional in that it inhibits ToMV multiplication without inducing a defense response. Several lines of evidence had suggested that Tm-1 encodes a direct inhibitor of ToMV RNA replication. The Tm-1 gene product was identified by purification of an inhibitor protein using a cell-free translation and replication system for ToMV RNA. Further analyses using the system showed that Tm-1 bound ToMV replication proteins, and that the Tm-1-bound ToMV replication proteins retained the ability to bind membranes, while Tm-1 inhibited replication complex formation on the membranes.