Fibrosarcoma versus cellular fibroma of the ovary - A comparative study of their proliferative activity and chromosome aberrations using MIB-1 immunostaining, DNA flow cytometry, and fluorescence in situ hybridization
Fibrosarcoma versus cellular fibroma of the ovary - A comparative study of their proliferative activity and chromosome aberrations using MIB-1 immunostaining, DNA flow cytometry, and fluorescence in situ hybridization
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DOI：
10.1097/00000478-199701000-00006
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发表时间：
1997-01-01
影响因子：
5.6
通讯作者：
Tsuneyoshi, M
中科院分区：
文献类型：
--
作者：
Tsuji, T;Kawauchi, S;Tsuneyoshi, M
我们回顾性分析了增殖活性和染色体的着丝粒拷贝数8，12，17在3例纤维肉瘤和8例卵巢细胞性纤维瘤使用MIB-1免疫染色，DNA流式细胞术，荧光原位杂交（FISH）石蜡包埋的组织标本。在我们的研究中，纤维肉瘤的MIB-1标记指数（LI）和增殖指数（S + G2 + M期细胞的百分比）均高于细胞性纤维瘤。FISH分析表明，在所有8例细胞性纤维瘤中，唯一的异常是12三体细胞的增加。1例纤维肉瘤同时出现12三体细胞和12四体细胞。在检测到信号的所有两个纤维肉瘤中观察到8三体细胞的增加。相比之下，在8例细胞性纤维瘤中，既没有观察到8三体细胞的增加，也没有观察到12四体细胞的增加。17号染色体均为二体性。在这些研究结果的基础上，因此认为8三体细胞的获得是区分卵巢细胞性纤维瘤和纤维肉瘤的充分有效的标志物，并且它也可能与卵巢纤维肉瘤的增殖活性有关。
We retrospectively analyzed the proliferative activity and the centrometric copy number of chromosomes 8, 12, and 17 in three cases of fibrosarcoma and eight cases of cellular fibroma of the ovary using MIB-1 immunostaining, DNA flow cytometry, and fluorescence in situ hybridization (FISH) on paraffin-embedded tissue specimens. In our study, both the MIB-1 labeling index (LI) and the proliferative index (% of cells in S + G2 + M phase) in fibrosarcomas were higher than those in cellular fibromas. The FISH analysis demonstrated the sole abnormality of a gain of trisomy 12 cells in all eight cases of cellular fibroma. Both a gain of trisomy 12 cells and a gain of tetrasomy 12 cells were observed in one case of fibrosarcoma. A gain of trisomy 8 cells was observed in all two fibrosarcomas in which signals were detected. By contrast, neither a gain of trisomy 8 cells nor again of tetrasomy 12 cells was observed in any of the eight cases of cellular fibroma. Chromosome 17 showed disomy in all eleven cases. In the basis of these findings, a gain of trisomy 8 cells is therefore considered to be an adequately effective marker to distinguish between cellular fibroma and fibrosarcoma of the ovary, and it may also be related to the proliferative activity of fibrosarcoma of the ovary.