Molecular epidemiological study of human rhinovirus species A, B and C from patients with acute respiratory illnesses in Japan
Molecular epidemiological study of human rhinovirus species A, B and C from patients with acute respiratory illnesses in Japan
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DOI：
10.1099/jmm.0.035006-0
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发表时间：
2012-03-01
影响因子：
3
通讯作者：
Kimura, Hirokazu
中科院分区：
文献类型：
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作者：
Arakawa, Mika;Okamoto-Nakagawa, Reiko;Kimura, Hirokazu
最近的研究表明，人类鼻病毒种类A、B和C （hrv - abc）可能与普通感冒和严重急性呼吸道疾病（ARIs）（如细支气管炎、喘息性细支气管炎和肺炎）有关。然而，这些病毒在日本的状态和分子流行病学尚不完全清楚。本研究检测了2010年1月至12月期间日本患者（92例，0-36岁，平均+/- SD 3.5 +/- 5.0岁）的HRV-ABCs基因组，这些患者患有包括上呼吸道感染、细支气管炎、喘息性细支气管炎、肺炎在内的各种急性呼吸道感染。根据病毒株间的两两距离临时划分hrv - abc型。根据VP4/VP2编码区的核苷酸序列，在系统进化树上，HRV-A、-B和-C分别被初步划分为14个、2个和12个类型。目前的HRV-A和-C株具有广泛的遗传多样性（约30%的差异）。基于核苷酸序列的种间距离分别为0.230 +/- 0.063（平均+/- SD， HRV-A）、0.218 +/- 0.048 （HRV-B）和0.281 +/- 0.105 (HRV-C)；基于推导出的氨基酸水平的种间距离分别为0.075 +/- 0.036 （HRV-A）、0.049 +/- 0.022 （HRV-B）和0.141 +/- 0.064 （HRV-C）。此外，HRV-A和hrv -C是优势种，在整个季节都有检测到。结果表明，HRV-A和-C株具有广泛的遗传差异，并与日本的各种ARIs有关。
Recent studies suggest that human rhinovirus species A, B and C (HRV-ABCs) may be associated with both the common cold and severe acute respiratory illnesses (ARIs) such as bronchiolitis, wheezy bronchiolitis and pneumonia. However, the state and molecular epidemiology of these viruses in Japan is not fully understood. This study detected the genomes of HRV-ABCs from Japanese patients (92 cases, 0-36 years old, mean +/- SD 3.5 +/- 5.0 years) with various ARIs including upper respiratory infection, bronchiolitis, wheezy bronchiolitis, croup and pneumonia between January and December 2010. HRV-ABCs were provisionally type assigned from the pairwise distances among the strains. On phylogenetic trees based on the nucleotide sequences of the VP4/VP2 coding region, HRV-A, -B and -C were provisionally assigned to 14, 2 and 12 types, respectively. The present HRV-A and -C strains had a wide genetic diversity (>30% divergence). The interspecies distances were 0.230 +/- 0.063 (mean +/- SD, HRV-A), 0.218 +/- 0.048 (HRV-B) and 0.281 +/- 0.105 (HRV-C), based on nucleotide sequences, and 0.075 +/- 0.036 (HRV-A), 0.049 +/- 0.022 (HRV-B) and 0.141 +/- 0.064 (HRV-C) at the deduced amino acid level. Furthermore, HRV-A and -C were the predominant species and were detected throughout the seasons. The results suggested that HRV-A and -C strains have a wide genetic divergence and are associated with various ARIs in Japan.