Hemoglobin A1C Percentage in Nonhuman Primates: A Useful Tool toMonitor Diabetes before and after Porcine Pancreatic Islet Xenotransplantation
Hemoglobin A1C Percentage in Nonhuman Primates: A Useful Tool toMonitor Diabetes before and after Porcine Pancreatic Islet Xenotransplantation
复制标题
DOI：
10.1155/2011/965605
复制
发表时间：
2011-01-01
影响因子：
2.5
通讯作者：
Bottino, Rita
中科院分区：
文献类型：
--
作者：
Marigliano, Marco;Casu, Anna;Bottino, Rita
非人灵长类动物（NHP）是一种非常有价值的糖尿病研究模型，包括实验性胰岛移植。特别是NHP是验证猪作为胰岛可能来源的首选受体。本研究的目的是量化NHP中糖化血红蛋白的百分比，并评估值的变化是否反映了糖尿病诱导和胰岛移植后的代谢趋势。分析了15名NHP的血清。9例NHP用链脲佐菌素（STZ）致糖尿病，其中3例接受猪胰岛移植。血红蛋白A1c（HbA1c）百分比采用基于乳胶免疫凝集抑制方法的测定法进行测量。糖尿病及其病程与HbA1c水平升高相关，而小组织移植后血糖正常化则被HbA1c百分比降低所阻碍。我们的数据提供了证据表明，HbA1c是一个有用的工具，以监测葡萄糖代谢的NHP。
Non-human primates (NHPs) are a very valuable experimental model for diabetes research studies including experimental pancreatic islet transplantation. In particular NHPs are the recipients of choice to validate pigs as possible source of pancreatic islets. The aim of this study was to quantify glycated hemoglobin percentage in NHPs and to assess whether changes in values reflect the metabolic trends after diabetes induction and islet transplantation. Sera from 15 NHPs were analyzed. 9 NHPs were rendered diabetic with streptozotocin (STZ), and 3 of them received porcine islet transplants. Hemoglobin A1c (HbA1c) percentage was measured with an assay based on a latex immunoagglutination inhibition methodology. Whereas diabetes and its duration were associated with increasing HbA1c levels, postislet transplantation blood glucose normalization was paralleled by a decrease in the HbA1c percentage. Our data provide evidence that HbA1c is a useful tool to monitor glucose metabolism in NHPs.