Medullary adrenergic neurons contribute to the neuropeptide Y-ergic innervation of hypophysiotropic thyrotropin-releasing hormone-synthesizing neurons in the rat.
Medullary adrenergic neurons contribute to the neuropeptide Y-ergic innervation of hypophysiotropic thyrotropin-releasing hormone-synthesizing neurons in the rat.
复制标题
髓质肾上腺素能神经元参与大鼠促垂体促甲状腺素释放激素合成神经元的神经肽 Y 能神经支配。
DOI：
10.1016/s0304-3940(02)00165-9
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发表时间：
2002
影响因子：
2.5
通讯作者：
Fekete,Csaba
中科院分区：
文献类型：
--
作者：
Wittmann,Gábor;Liposits,Zsolt;Lechan,RonaldM;Fekete,Csaba
弓形核产生约80%的神经肽Y (NPY)-免疫反应（IR）神经支配下丘脑室旁核（PVN）的促甲状腺素释放激素（TRH）神经元。然而，其余20%的来源是未知的。由于髓质肾上腺素能神经元能合成NPY并支配垂体性TRH神经元，因此我们提出肾上腺素能神经元参与PVN中TRH神经元的NPY能支配的可能性。采用三标记免疫荧光法研究NPY和苯乙醇胺n -甲基转移酶（phenyethanol amine N-methyltransferase， PNMT）（肾上腺素合成的关键酶）在与垂体性TRH神经元相关的轴突中的共定位。在74%的PNMT-IR轴突变异中观察到npy -免疫反应性，其中26%的NPY-IR轴突与PVN的核周和树突接触。我们得出结论，至少有两种不同的NPY神经元群支配着嗜垂体TRH神经元，下丘脑弓状核的NPY神经元和共同含有NPY的髓质肾上腺素能神经元。
The arcuate nucleus gives rise to approximately 80% of the neuropeptide Y (NPY)-immunoreactive (IR) innervation to thyrotropin-releasing hormone (TRH) neurons in the hypothalamic paraventricular nucleus (PVN). However, the source of the remaining 20% is unknown. Since medullary adrenergic neurons synthesize NPY and also innervate the hypophysiotropic TRH neurons, we raised the possibility that adrenergic neurons contribute to the NPY-ergic innervation of TRH neurons in the PVN. Triple-labeling immunofluorescence was performed to study the colocalization of NPY and phenylethanolamine N-methyltransferase (PNMT)—the key enzyme of adrenaline synthesis—in axons in association with hypophysiotropic TRH neurons. NPY-immunoreactivity was observed in 74% of PNMT-IR axon varicosities apposed to proTRH-IR neurons, comprising 26% of all NPY-IR axons in contact with proTRH perikarya and dendrites in the PVN. We conclude that at least two distinct populations of NPY neurons innervate hypophysiotropic TRH neurons, the NPY neurons of the hypothalamic arcuate nucleus and the medullary adrenergic neurons that co-contain NPY.