Quantitative proteomics reveals ecological fitness cost of multi-herbicide resistant barnyardgrass (Echinochloa crus-galli L.)
Quantitative proteomics reveals ecological fitness cost of multi-herbicide resistant barnyardgrass (Echinochloa crus-galli L.)
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定量蛋白质组学揭示了多重除草剂抗性稗草 (Echinochloa crus-galli L.) 的生态适应性成本
DOI：
10.1016/j.jprot.2016.09.009
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发表时间：
2017-01-06
影响因子：
3.3
通讯作者：
Li, Yongfeng
中科院分区：
文献类型：
--
作者：
Yang, Xia;Zhang, Zichang;Li, Yongfeng
稗草（Echinochloa crus-galli）是世界上15种抗除草剂杂草之一，它对水稻的生长具有很大的干扰作用，严重影响水稻的产量。因此，多除草剂抗性稗草提出了一个主要的威胁，与潜在的机制，有助于电阻需要阐明。为了在蛋白质组学水平上表征这种多除草剂抗性，使用iTRAQ在有和没有二氯喹啉酸、双草醚钠和五氟磺草胺除草剂处理的情况下对抗性和敏感稗草进行比较分析。通过检索UniProt数据库并进行数据分析，从2248个独特的肽中鉴定出总共1342个蛋白质种类。大约904种蛋白质种类和4774个基因本体论（GO）术语被分为生物过程、细胞成分和分子功能类别。其中，688种蛋白质被注释到1583条KEGG通路中，其中980种蛋白质与代谢有关，93种蛋白质与环境信息处理有关。共有292种蛋白质种类显示出超过1.2倍的变化，相对于敏感的生物型的丰富的抗性生物型。此外，除草剂处理导致了157种蛋白质，表现出超过1.2倍的变化，在抗性生物型。此外，生理分析表明，在抗性biotype.Significance的生态健身成本：虽然一些研究表明，健身成本与改变生态相互作用，我们的理解与除草剂抗性的健身成本是有限的。本文通过生理和蛋白质组学分析，揭示了E.我的老天爷本文的结果揭示了E.并提供了一个基本的基础，使杂草控制的新策略的发展。最后，这是第一个大规模的蛋白质组学研究，以研究不同稗草生物型的除草剂胁迫反应。(C)2016爱思唯尔B.V.保留所有权利。
Barnyardgrass (Echinochloa crus-galli) is one of the top 15 herbicide-resistant weeds around the world that interferes with rice growth, resulting in major losses of rice yield. Thus, multi-herbicide resistance in barnyardgrass presents a major threat, with the underlying mechanisms that contribute to resistance requiring elucidation. In an attempt to characterize this multi-herbicide resistance at the proteomic level, comparative analysis of resistant and susceptible barnyardgrasses was performed using iTRAQ both with and without quinclorac, bispyribac-sodium and penoxsulam herbicidal treatment. A total of 1342 protein species were identified from 2248 unique peptides by searching the UniProt database and conducting data analysis. Approximately 904 protein species with 4774 Gene Ontology (GO) terms were grouped into the categories of biological process, cellular component and molecular function. Among these, 688 protein species were annotated into 1583 KEGG pathways, with 980 protein species relating to metabolism and 93 relating to environmental information processing. A total of 292 protein species showed more than a 1.2-fold change in abundance in the resistant biotype relative to the susceptible biotype. Furthermore, herbicide treatment resulted in 157 protein species that showed more than a 1.2-fold change in the resistant biotype. Moreover, physiological analyses demonstrated an ecological fitness cost in the resistant biotype.Significance: While some studies have shown a fitness cost to be associated with an altered ecological interaction, our understanding of the fitness costs associated with herbicide resistance are limited. Herein, physiological and proteomic analysis demonstrates herbicide resistance associated ecological fitness cost and potential mechanisms of herbicide-resistance in resistant biotypes of E. crus-galli. The results presented herein have revealed differences in ecological adaptation between resistant and susceptible biotypes in E. crus-galli and provide a fundamental basis enabling the development of new strategies for weed control. Lastly, this is the first large-scale proteomics study to examine herbicide stress responses in different barnyardgrass biotypes. (C) 2016 Elsevier B.V. All rights reserved.