Comparative morphological examination of vertebral bodies of teleost fish using high‐resolution micro‐CT scans
Comparative morphological examination of vertebral bodies of teleost fish using high‐resolution micro‐CT scans
复制标题
使用高分辨率显微 CT 扫描对硬骨鱼椎体进行比较形态学检查
DOI：
10.1002/jmor.20983
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发表时间：
2019
影响因子：
1.5
通讯作者：
Kondo Shigeru
中科院分区：
文献类型：
--
作者：
Sakashita Misaki;Sato Mao;Kondo Shigeru
硬骨鱼的脊椎体是由覆盖在脊索中心上的骨节骨形成的。巩膜骨的内部由一个双凹的沙漏状的自体组成，这在大多数鱼类中是常见的。相比之下，巩膜骨的外部形状在物种之间变化很大。关于外部结构的组成和形成有多种假设。然而，由于它们是基于对少数现存或灭绝物种的研究，它们对其他物种的适用性仍有待澄清。为了了解硬骨鱼类椎体的形态、形成和组成，我们使用显微CT扫描对来自10个硬骨鱼目的32个物种进行了比较分析，并研究了巩膜骨的详细形态，特别是其板状脊和小梁。我们发现了两个结构特征，大多数被检查的物种之间的共享。一种是片状骨小梁，从椎体中心径向延伸，厚度恒定。另一种是在侧嵴和骨小梁的内部存在空腔。不同排列的片状骨小梁和内部中空空间的组合形成了不同形状的椎体外侧结构。这两个特征的性质表明，通过在骨尖处沉积，巩膜骨的外部向外生长，并且同时，骨吸收发生在巩膜骨的内部。椎弓也由片状小梁形成，表明椎体和椎弓由相同的成分形成。微CT扫描数据被上传到公共数据库，以便将来用于鱼类脊椎的研究。
Vertebral bodies of teleost fish are formed by the sclerotomal bone covering the chordacentrum. The internal part of the sclerotomal bone is composed of an amphicoelous hourglass shaped autocentrum, which is common in most fish species. In contrast, the external shape of the sclerotomal bone varies extensively among species. There are multiple hypotheses regarding the composition and formation of the external structure. However, as they are based on studies of few extant or extinct species, their applicability to other species remains to be clarified. To understand the morphology, formation, and composition of vertebral bodies in teleosts, we performed a comparative analysis using micro‐CT scans of 32 species from 10 orders of Teleostei and investigated the detailed morphology of the sclerotomal bone, especially its plate‐like ridge and trabeculae. We discovered two structural characteristics that are shared among most of the examined species. One was the sheet‐like trabeculae that extend radially from the center of the vertebral body with a constant thickness. The other was the presence of hollow spaces on the internal parts of the lateral ridge and trabeculae. The combination of different arrangements of sheet‐like trabeculae and internal hollow spaces formed different shapes of the lateral structure of the vertebral body. The properties of these two characteristics suggest that the external part of the sclerotomal bone grows outward by deposition at the bone tip, and that, concurrently, bone absorption occurs in the internal part of the sclerotomal bone. The vertebral arches were also formed by the sheet‐like trabeculae, indicating that both, the vertebral body and the arches, are formed by the same component. The micro‐CT scanning data were uploaded to a public database so they can be used for future studies on fish vertebrae.