Resolvin D1 modulates periodontal ligament fibroblast function.
Resolvin D1 modulates periodontal ligament fibroblast function.
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Resolvin D1 调节牙周膜成纤维细胞功能。
DOI：
10.1002/jper.22-0462
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发表时间：
2023
影响因子：
4.3
通讯作者：
Kantarci,Alpdogan
中科院分区：
文献类型：
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作者：
Zarrough,AhmedE;Hasturk,Hatice;Stephens,DanielleN;VanDyke,ThomasE;Kantarci,Alpdogan
背景炎症的消退是一个由脂氧素和消退素介导的主动过程。牙周膜成纤维细胞（PDLFs）在牙周再生中起着重要作用。本研究的目的是确定Resolvin D1（RvD 1）对人PDLF细胞伤口愈合和增殖、受体表达（G蛋白偶联受体32 [GPR 32]和甲酰肽受体2 [ALX/FPR 2]）以及细胞因子表达和释放的影响。通过定量真实的时间聚合酶链反应（qPCR）、免疫荧光显微镜和荧光激活细胞分选术测定RvD 1受体表达。通过划痕试验测量伤口闭合，通过溴脱氧尿苷掺入测定增殖。白细胞介素-6（IL-6）、白细胞介素-8（IL-8）、单核细胞趋化蛋白-1、环氧合酶-2、基质金属蛋白酶-1、-2和-3（MMP-1，-2和-3），金属蛋白酶-1和-2的组织抑制剂使用qPCR和蛋白质印迹法、多重免疫测定法，结果PDLF表达GPR 32和ALX/FPR 2。RvD 1逆转IL-1β诱导的对伤口愈合和PDLF增殖的抑制。IL-1β还诱导促炎细胞因子和MMP的产生。这种效应被RvD 1逆转。RvD 1逆转了IL-1β 1诱导的TIMP-1、TIMP-2和OPG的抑制作用。结论数据表明，RvD 1对PDLF具有促伤口愈合、增殖和抗炎作用，有利于牙周再生。
BackgroundThe resolution of inflammation is an active process mediated by specialized lipid mediators called lipoxins and resolvins. Periodontal ligament fibroblasts (PDLFs) play a significant role in periodontal regeneration. The purpose of the current study was to determine the impact of resolvin D1 (RvD1) on human PDLF cell wound healing and proliferation, receptor expression (G‐protein‐coupled receptor 32 [GPR32] and formyl peptide receptor 2 [ALX/FPR2]), and cytokine expression and release.MethodsPDLFs were stimulated with interleukin‐1β (IL‐1β) (500 pg/ml) with and without RvD1 (100 nM). RvD1 receptor expression was determined by quantitative real‐time polymerase chain reaction (qPCR), immunofluorescence microscopy, and fluorescence‐activated cell sorting. Wound closure was measured by a scratch assay, and proliferation was determined by bromodeoxyuridine incorporation. Interleukin‐6 (IL‐6), interleukin‐8 (IL‐8), monocyte chemoattractant protein‐1, cyclooxygenase‐2, matrix metalloproteinases‐1, ‐2, and ‐3 (MMP‐1, ‐2, and ‐3), tissue inhibitors of metalloproteinases‐1 and ‐2 (TIMP‐1 and ‐2), prostaglandin E2, and osteoprotegerin (OPG) gene expression and production were measured using qPCR and Western blotting, multiplex immunoassay, and enzyme‐linked immunosorbent assay.ResultsPDLF expressed GPR32 and ALX/FPR2. RvD1 reversed IL‐1β‐induced inhibition of wound healing and proliferation of PDLF. IL‐1β also induced the production of proinflammatory cytokines and MMPs. This effect was reversed by RvD1. RvD1 reversed IL‐1β‒induced inhibition of TIMP‐1, TIMP‐2, and OPG.ConclusionThe data suggested that RvD1 has a pro‐wound healing, proliferative, and anti‐inflammatory impact on the PDLF that favors periodontal regeneration.