Microfluidic impedance cytometry of tumour cells in blood.
Microfluidic impedance cytometry of tumour cells in blood.
复制标题
DOI：
10.1063/1.4904405
复制
发表时间：
2014-12
期刊：
影响因子：
3.2
通讯作者：
D. Spencer;Veronica Hollis;H. Morgan
中科院分区：
文献类型：
--
作者：
D. Spencer;Veronica Hollis;H. Morgan
已知肿瘤细胞的介电特性与正常血细胞不同，并且可以利用这种差异进行细胞的无标记分离。传统的测量技术速度缓慢，并且无法在现实的时间范围内识别罕见的循环肿瘤细胞（CTC）。我们使用高通量单细胞微流体阻抗细胞术来测量 MCF7 肿瘤细胞系（CTC 的代表）的介电特性，无论是纯群体还是与全血混合。数据显示，MCF7 细胞具有较大的膜电容和尺寸，能够与所有其他白细胞清楚地区分。阻抗分析用于跟踪细胞悬浮时细胞活力的变化，这一过程可以通过对细胞介电特性（主要是膜电导率）随时间的变化进行建模来理解。阻抗细胞术用于计数掺入全血中的少量 MCF7 细胞。化学裂解通常用于去除丰富的红细胞，并且表明该过程不会改变 MCF7 细胞计数或改变其介电特性。将阻抗细胞术与基于磁珠的抗体富集相结合，可以在 1 ml 全血中检测到 100 个 MCF7 细胞，富集度为 log 3.5，平均回收率为 92%。微流控阻抗细胞术可以轻松集成到复杂的细胞分离系统中，用于特定细胞类型的识别和计数，提供快速的在线单细胞表征方法。
The dielectric properties of tumour cells are known to differ from normal blood cells, and this difference can be exploited for label-free separation of cells. Conventional measurement techniques are slow and cannot identify rare circulating tumour cells (CTCs) in a realistic timeframe. We use high throughput single cell microfluidic impedance cytometry to measure the dielectric properties of the MCF7 tumour cell line (representative of CTCs), both as pure populations and mixed with whole blood. The data show that the MCF7 cells have a large membrane capacitance and size, enabling clear discrimination from all other leukocytes. Impedance analysis is used to follow changes in cell viability when cells are kept in suspension, a process which can be understood from modelling time-dependent changes in the dielectric properties (predominantly membrane conductivity) of the cells. Impedance cytometry is used to enumerate low numbers of MCF7 cells spiked into whole blood. Chemical lysis is commonly used to remove the abundant erythrocytes, and it is shown that this process does not alter the MCF7 cell count or change their dielectric properties. Combining impedance cytometry with magnetic bead based antibody enrichment enables MCF7 cells to be detected down to 100 MCF7 cells in 1 ml whole blood, a log 3.5 enrichment and a mean recovery of 92%. Microfluidic impedance cytometry could be easily integrated within complex cell separation systems for identification and enumeration of specific cell types, providing a fast in-line single cell characterisation method.