Abscisic acid and hydrogen peroxide are involved in drought priming-induced drought tolerance in wheat (Triticum aestivum L.).
Abscisic acid and hydrogen peroxide are involved in drought priming-induced drought tolerance in wheat (Triticum aestivum L.).
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DOI：
10.1111/plb.13143
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发表时间：
2020-06
期刊：
影响因子：
3.9
通讯作者：
Xiao Wang;J. Zhang;Jiamin Song;Mei Huang;J. Cai;Qin Zhou;T. Dai;D. Jiang
中科院分区：
文献类型：
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作者：
Xiao Wang;J. Zhang;Jiamin Song;Mei Huang;J. Cai;Qin Zhou;T. Dai;D. Jiang
干旱是影响全球小麦生产的主要胁迫因素之一。生育早期干旱诱导可以提高小麦的抗旱性。脱落酸（ABA）和过氧化氢（H2 O2）是植物适应干旱胁迫的重要信号分子。然而，ABA和h2o2在干旱诱导抗旱性中的作用尚不清楚。本研究通过分析小麦对ABA抑制剂、H2清除剂和H2抑制剂的响应，探讨(1)ABA和H2在营养期干旱诱导后渗透调节中的关系，以及(2)灌浆期对干旱胁迫的响应。在单纯干旱胁迫处理中，化学处理导致ABA和H2 O2的清除，减弱了干旱胁迫的缓解作用，表现为叶片水势降低，籽粒产量下降。ABA抑制剂完全抑制了ABA和H2 O2的积累，抑制了呼吸爆发氧化酶同源物的表达，而H2 O2抑制剂导致9-顺式环氧类胡萝卜素双加氧酶的表达和ABA浓度升高，表明ABA清除抑制了H2 O2的生物合成，而H2 O2清除不抑制ABA的生物合成。结果进一步表明，NADPH氧化酶介导的H2生成作用于ABA的下游，从而诱导渗透转录物的表达和积累，从而有助于干旱诱导的胁迫耐受。这些结果为更好地理解小麦植株干旱诱导耐受的机制提供了理论基础。
Drought is one of the major stress factors in wheat production on a global scale. Drought priming during the early growth stage can enhance drought tolerance in wheat (Triticum aestivum L.). Abscisic acid (ABA) and hydrogen peroxide (H2 O2 ) are important signal molecules in the adaptation of plants to drought stress. However, the roles of ABA and H2 O2 in drought priming-induced drought tolerance are not clear. In the present study, we evaluated the responses of wheat to an ABA inhibitor, H2 O2 scavenger and inhibitor to investigate the (1) relationship between ABA and H2 O2 in osmotic adjustment after drought priming in the vegetative stage and (2) responses to drought stress during grain filling. In the drought priming-only treatments, chemical application resulted in the scavenging of ABA and H2 O2 , weakening the alleviation effects of drought priming on drought stress, as demonstrated by the lower leaf water potential and grain yield. The ABA inhibitor completely inhibited the accumulation of ABA and H2 O2 , ABA inhibitor inhibited the respiratory burst oxidase homologs expression, whereas the H2 O2 inhibitor resulted in a higher 9-cis-epoxycarotenoid dioxygenase expression and ABA concentration in primed plants, indicating that ABA scavening inhibited H2 O2 biosynhtesis while H2 O2 scavenging did not inhibit ABA biosynthesis. The results further demonstrated that NADPH oxidase-mediated H2 O2 production functions downstream of ABA, and this induces osmolyte transcript expression and accumulation and thus contributes to drought priming-induced stress tolerance. These results provide a theoretical basis for a better understanding of the mechanisms involved in drought priming-induced tolerance in wheat plants.