Arginine-modified DNA aptamers that show enantioselective recognition of the dicarboxylic acid moiety of glutamic acid
Arginine-modified DNA aptamers that show enantioselective recognition of the dicarboxylic acid moiety of glutamic acid
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DOI：
10.2116/analsci.24.167
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发表时间：
2008-01-01
影响因子：
1.6
通讯作者：
Sawai, Hiroaki
中科院分区：
文献类型：
--
作者：
Ohsawa, Kazuomi;Kasamatsu, Toshiyuki;Sawai, Hiroaki
我们已经筛选谷氨酸结合适体从修饰的DNA池含有精氨酸残基使用配体的系统进化指数富集（SELEX）的方法。在第17轮选择后，从富集池中获得31个修饰的DNA分子，并通过使用亲和凝胶的结合测定来评估它们对靶标的结合亲和力。三个修饰的DNA分子具有更高的亲和力进行测序，我们确定了它们的亲和力和特异性的目标，通过表面等离子体共振（SPR）测量。SPR研究表明，这三种适体中的两种将D-异构体的二羧酸部分与L-异构体的二羧酸部分区分开;然而，第三种适体没有显示出对映选择性。
We have screened glutamic acid-binding aptamers from a modified DNA pool containing arginine residues using the method of systematic evolution of ligands by exponential enrichment (SELEX). Thirty-one modified DNA molecules were obtained from the enriched pool after the 17th round of selection, and their binding affinities for the target were evaluated by binding assays using affinity gels. Three modified DNA molecules having higher affinity were sequenced and we determined their affinity and specificity for the target by surface plasmon resonance (SPR) measurements. The SPR studies indicated that two of these three aptamers distinguished the dicarboxylic acid moiety of the D-isomer from that of the L-isomer; however, the third aptamer did not show enantioselectivity.