Rab5 regulates motility of early endosomes on microtubules
Rab5 regulates motility of early endosomes on microtubules
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DOI：
10.1038/14075
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发表时间：
1999-10-01
影响因子：
21.3
通讯作者：
Zerial, M
中科院分区：
文献类型：
--
作者：
Nielsen, E;Severin, F;Zerial, M
小GTPase Rab5在早期内吞途径中调控膜对接和融合。在这里，我们揭示了Rab5在调节内核体与微管网络相互作用中的新作用。利用Rab5与绿色荧光蛋白的融合，我们发现Rab5阳性的核内体在体内微管上移动。在体外，Rab5既刺激早期核内体与微管的结合，又刺激早期核内体向微管负端的运动。此外，与核内体膜对接和融合类似，rab5依赖的核内体运动依赖于磷脂酰肌醇-3- oh激酶hVPS34。因此，Rab5在功能上与早期核内体的膜运输、运动和细胞内分布的调节有关。
The small GTPase Rab5 regulates membrane docking and fusion in the early endocytic pathway. Here we reveal a new role for Rab5 in the regulation of endosome interactions with the microtubule network. Using Rab5 fused to green fluorescent protein we show that Rab5-positive endosomes move on microtubules in vivo. In vitro, Rab5 stimulates both association of early endosomes with microtubules and early-endosome motility towards the minus ends of microtubules. Moreover, similarly to endosome membrane docking and fusion, Rab5-dependent endosome movement depends on the phosphatidylinositol-3-OH kinase hVPS34. Thus, Rab5 functionally links regulation of membrane transport, motility and intracellular distribution of early endosomes.