dTrf2 is required for transcriptional and developmental responses to ecdysone during Drosophila metamorphosis
dTrf2 is required for transcriptional and developmental responses to ecdysone during Drosophila metamorphosis
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DOI：
10.1002/dvdy.21350
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发表时间：
2007-11-01
影响因子：
2.5
通讯作者：
Thummel, Carl S.
中科院分区：
文献类型：
--
作者：
Bashirullah, Arash;Lam, Geanette;Thummel, Carl S.
TATA盒结合蛋白（TBP）相关因子2 （TRF2）已经在生化水平和培养细胞中得到了很好的表征。然而，对于TRF2在发育过程中如何在特定的生物学途径中发挥作用，我们所知相对较少。在这里，我们发现果蝇TRF2 （dTRF2）在变态开始时对类固醇激素蜕皮激素的反应中起重要作用。半胚性dTrf2突变导致蛹前和蛹早期发育停滞，并导致主要蜕皮激素触发的生物反应缺陷，包括蛹形成、前气门外翻、气泡易位、成虫头部外翻和幼虫唾液腺细胞死亡。在dTrf2突变体中，关键的表皮激素调控靶基因的转录被延迟和减少。dTrf2似乎是进入变态所需的蜕皮激素调节基因表达的适当时机和水平所必需的。
The TATA box-binding protein (TBP) related factor 2 (TRF2) has been well characterized at a biochemical level and in cultured cells. Relatively little, however, is known about how TRF2 functions in specific biological pathways during development. Here, we show that Drosophila TRF2 (dTRF2) plays an essential role in responses to the steroid hormone ecdysone during the onset of metamorphosis. Hypomorphic dTrf2 mutations lead to developmental arrest during prepupal and early pupal stages with defects in major ecdysone-triggered biological responses, including puparium formation, anterior spiracle eversion, gas bubble translocation, adult head eversion, and larval salivary gland cell death. The transcription of key ecdysone-regulated target genes is delayed and reduced in dTrf2 mutants. dTrf2 appears to be required for the proper timing and levels of ecdysone-regulated gene expression required for entry into metamorphosis.