Mycoplasma pneumoniae induces allergy by producing P1-specific immunoglobulin E
Mycoplasma pneumoniae induces allergy by producing P1-specific immunoglobulin E
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肺炎支原体通过产生P1特异性免疫球蛋白E诱发过敏
DOI：
10.1016/j.anai.2018.03.014
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发表时间：
2018-07-01
影响因子：
5.9
通讯作者：
Shang, Shi-qiang
中科院分区：
文献类型：
--
作者：
Ye, Qing;Mao, Jian-hua;Shang, Shi-qiang
背景：我们前期研究发现大多数肺炎支原体肺炎（MPP）患者血清总免疫球蛋白E（IgE）水平升高。目的：明确导致儿童IgE升高的MP成分，并阐明其具体机制。方法：通过MP肺炎患者血清IgE分离MP细胞成分。将这些通过原核表达获得的成分作为过敏原，检测MPP患者体内产生的过敏原特异性IgE的比例，并分析产生这种过敏原特异性IgE的患者的临床特征和相关免疫参数。此外，还通过细胞实验验证了这些成分的体外生物学效应。结果：MPP儿童血清中检测到PI特异性IgE。通过镍琼脂糖亲和层析纯化，原核表达获得约24 kDa的P1蛋白多肽。大约9.2%的MPP患者产生针对P1蛋白的这种多肽的IgE，这种IgE更有可能在没有过敏史或过敏相关疾病家族史的MPP患者中产生。 P1特异性IgE阳性MPP患者临床症状更严重，白介素（IL）-4和IL-5分泌过多，Th0细胞过度分化为Th2细胞。测试还表明，P1蛋白刺激健康供体外周血的外周血单核细胞过度分泌IL-4和IL-5。 结论：肺炎支原体不仅是一种感染原，而且对某些个体来说也是一种过敏原。 MP的P1蛋白可以诱导P1特异性IgE的产生。 (C) 2018 年美国过敏、哮喘和免疫学学院。由爱思唯尔公司出版。保留所有权利。
Background: Our previous study found that most Mycoplasina pneurnoniae (MP) pneumonia (MPP)patients had elevated serum total immunoglobulin E (IgE) levels.Objective: To determine components of MP that can cause an IgE increase in children, and to clarify its specific mechanism.Methods: The components of MP cells were isolated by serum IgE from patients with MP pneumonia. These components obtained through the prokaryotic expression were used as allergens to detect the proportion of allergen-specific IgE produced in MPP patients, and the clinical characteristics and related immune parameters of these patients who produced this allergen-specific IgE were also analyzed. In addition, a cell experiment was used to verify the biological effect of these components in vitro.Results: Pl-specific IgE was detected in serum of MPP children. An approximately 24-kDa polypeptide of P1 protein was obtained through prokaryotic expression purified by nickel agarose affinity chromatography. Approximately 9.2% of MPP patients produced IgE against this polypeptide of P1 protein, which was more likely to be produced in MPP patients with no history of allergies or family history of allergy-related diseases. P1-specific IgE-positive MPP patients had more severe clinical symptoms, with excessive secretion of interleukin (IL)-4 and IL-5 and overdifferentiation of Th0 cells into Th2 cells. Tests also demonstrated that the P1 protein stimulated excessive secretion of IL-4 and IL-5 in peripheral blood mononuclear cells from the peripheral blood of healthy donors.Conclusion: Mycoplasnia pneuntonine is not only an infectious agent but also an allergen for certainindividuals. The P1 protein of MP can induce the production of Pl-specific IgE. (C) 2018 American College of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.