Kisspeptin2 regulates hormone expression in female zebrafish (Danio rerio) pituitary
Kisspeptin2 regulates hormone expression in female zebrafish (Danio rerio) pituitary
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Kisspeptin2 调节雌性斑马鱼 (Danio rerio) 垂体的激素表达
DOI：
10.1016/j.mce.2020.110858
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发表时间：
2020
影响因子：
4.1
通讯作者：
Wei Hu
中科院分区：
文献类型：
--
作者：
Yanlong Song;Ji Chen;Binbin Tao;Daji Luo;Zuoyan Zhu;Wei Hu
Kisspeptin2是一种神经肽，广泛存在于斑马鱼的脑和多种外周组织中。脑下垂体是合成的中心，分泌各种内分泌激素。然而，Kiss2在斑马鱼脑下垂体中的神经支配尚不清楚。在本研究中，我们研究了启动子驱动的mCherry标记的Kiss2神经元对斑马鱼脑垂体中Kiss2细胞和结构的组织。下丘脑中的Kiss2神经元不会投射到脑下垂体。Kiss2细胞存在于雌性脑垂体部。Kiss2细胞和突起位于近端远侧部，类似于GnRH3纤维的分布。Kiss2结构位于GnRH3纤维旁边。雄性垂体腺中未发现Kiss2结构。Kispeptin受体Kiss1rb在雌性和雄性脑下垂体中都有转录表达。原位杂交显示Kiss1rb阳性细胞位于PPD和中间部(PI)。在体外，Kiss2-10处理可刺激Akt和Erk的磷酸化，并显著诱导雌性脑垂体促黄体生成素β、促卵泡刺激素β和prl1mRNA的表达。本研究结果提示，在雌性斑马鱼脑垂体中，Kiss2和Kiss1rb可能形成一个独立的旁分泌或自分泌系统。Kiss2和Kiss1rb信号调节垂体激素的表达。
Kisspeptin2 is a neuropeptide widely found in the brain and multiple peripheral tissues in the zebrafish. The pituitary is the center of synthesis and secretes various endocrine hormones. However, Kiss2 innervation in the zebrafish pituitary is unknown. In this study, the organization of Kiss2 cells and structures in the zebrafish pituitary by promoter-driving mCherry-labeling Kiss2 neurons were investigated. Kiss2 neurons in the hypothalamus do not project into the pituitary. Kiss2 cells are found in the female pituitary. Kiss2 cells and extensions are located in the proximal pars distalis (PPD), similar to the distribution of Gnrh3 fibers. Kiss2 structures reside alongside Gnrh3 fibers. No Kiss2 structures are found in the male pituitary. The transcriptional expression of the kisspeptin receptor kiss1rb is detected in both female and male pituitaries. In situ hybridization shows that kiss1rb-positive cells are located in the PPD and pars intermedia (PI). In vitro Kiss2-10 treatment stimulates Akt and Erk phosphorylation and significantly induces lhβ, fshβ, and prl1 mRNA expression in the female pituitary. The results in this study suggest that Kiss2 and Kiss1rb may form an independent paracrine or autocrine system in the female zebrafish pituitary. Kiss2 and Kiss1rb signaling regulates the expression of pituitary hormones.