Very late activation antigens (VLA) are human leukocyte-neuronal crossreactive cell surface antigens.
Very late activation antigens (VLA) are human leukocyte-neuronal crossreactive cell surface antigens.
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DOI：
10.1084/jem.164.2.393
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发表时间：
1986-08-01
期刊：
影响因子：
--
通讯作者：
Woods VL Jr
中科院分区：
文献类型：
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作者：
Pischel KD;Bluestein HG;Woods VL Jr
用抗人外周血单个核细胞（PBMC）的异源抗血清分析了人神经细胞和淋巴细胞表面抗原之间的抗原关系。交叉反应性抗原的特异性通过使用兔抗PBMC（RAPBMC）血清的125 I标记的SK-N-SH培养的神经元细胞的免疫沉淀来检查，并与使用mAb的已知特异性进行比较。检测的各兔抗血清对SK-N-SH细胞的主要反应性是与130，000、160，000和180，000 Mr的三个分子。这三条链与用极晚期活化抗原（VLA）特异性mAb A-1A 5沉淀的三个分子共迁移。用mAb A-1A 5进行的顺序沉淀确定了三个RAPBMC沉淀的条带是VLA复合物的成员。这通过RAPBMC和A-1A 5免疫沉淀物的二维PAGE证实，它们彼此无法区分。二维模式比从VLA链缔合的异二聚体模型中预期的更复杂，并表明VLA的额外130，000 Mr组分。由RAPBMC或mAb A-1A 5从SK-N-SH神经元沉淀的VLA复合物的三条链与活化的T细胞上存在的VLA模式非常相似，包括由mAb TS 2/7识别的180，000 Mr活化特异性α 1链。正常脑细胞膜也含有由RAPBMC和mAb A-1A 5沉淀的VLA分子。因此，VLA复合物在人类神经元和T细胞上提供了潜在的重要的共享免疫原。
The antigenic relationship between human neuronal and lymphocyte cell surface antigens has been analyzed using heteroantisera raised against human peripheral blood mononuclear cells (PBMC). The specificities of the crossreactive antigens were examined by immunoprecipitation of 125I- labeled SK-N-SH cultured neuronal cells using rabbit anti-PBMC (RAPBMC) sera and compared to known specificities using mAb. The predominant reactivity of each rabbit antiserum tested against SK-N-SH cells was with three molecules of 130,000, 160,000, and 180,000 Mr. These three chains comigrated with three molecules precipitated with the very late activation antigen (VLA)-specific mAb A-1A5. Sequential precipitations with mAb A-1A5 established that the three RAPBMC-precipitated bands were members of the VLA complex. This was confirmed by two-dimensional PAGE of the RAPBMC and A-1A5 immunoprecipitates, which were indistinguishable from one another. The two-dimensional pattern was more complex than was anticipated from the heterodimeric model of VLA chain association, and suggests an additional 130,000 Mr component of VLA. The three chains of the VLA complex precipitated by RAPBMC or mAb A-1A5 from SK-N-SH neurons closely resembled the VLA pattern present on activated T cells, including the 180,000 Mr activation-specific alpha 1 chain recognized by mAb TS2/7. Normal brain cell membranes also contain VLA molecules that are precipitated by RAPBMC and mAb A-1A5. Thus the VLA complex provides potentially important shared immunogens on human neurons and T cells.