Phylogenetic Identification of Hypermastigotes, Pseudotrichonympha, Spirotrichonympha, Holomastigotoides, and Parabasalian Symbionts in the Hindgut of Termites
Phylogenetic Identification of Hypermastigotes, Pseudotrichonympha, Spirotrichonympha, Holomastigotoides, and Parabasalian Symbionts in the Hindgut of Termites
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DOI：
10.1111/j.1550-7408.2000.tb00044.x
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发表时间：
2000-05
期刊：
影响因子：
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通讯作者：
M. Ohkuma;Kuniyo Ohtoko;T. Iida;M. Tokura;S. Moriya;R. Usami;K. Horikoshi;T. Kudo
中科院分区：
文献类型：
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作者：
M. Ohkuma;Kuniyo Ohtoko;T. Iida;M. Tokura;S. Moriya;R. Usami;K. Horikoshi;T. Kudo
摘要根据直接从低等白蚁后肠鞭毛虫混合种群中扩增出的小亚单位核糖体RNA基因序列，研究了副寄生虫鞭毛虫的系统发育多样性。总共从8种白蚁中回收了33个具有代表性的副白蚁序列。设计了针对特定序列的荧光标记寡核苷酸探针，并用于全细胞杂交原位鉴定副寄生虫物种。台湾毛白蚁和台湾毛白蚁后肠中的超乳殖子--格雷氏拟毛虫、莱氏螺旋白蚁和大腹乳突白蚁。和毛环菌(Trichonympha spp.)在HODOTERMEROMPIS sjostedti中鉴定出。在构建的系统发育树中，白蚁的序列分散在白蚁的已知成员群中，反映了白蚁后肠中存在着不同的副珊瑚。以伪毛虫、毛毛虫和螺毛虫为代表的三个超级受精卵的家系与基于形态的分类群一致。对树根的分析表明，伪毛蜂群是副石龙科最可能的古老谱系，毛蜂群是第二个深分支的谱系。螺旋毛滴虫和毛滴虫的出现时间可能较晚。
Abstract The phylogenetic diversity of parabasalian flagellates was examined based on the sequences of small subunit ribosomal RNA genes amplified directly from the mixed population of flagellates in the hindgut of lower termites. In total, 33 representative sequences of parabasalids were recovered from eight termite species. Fluorescent-labeled oligonucleotide probes specific for certain sequences were designed and used for the in situ identification of parabasalian species by whole-cell hybridization. The hypermastigotes, Pseudotrichonympha grassii, Spirotrichonympha leidyi, and Holomastigotoides mirabile in the hindgut of Coptotermes formosanus, and Spirotrichonympha sp. and Trichonympha spp. in Hodotermopsis sjoestedti were identified. In the phylogenetic tree constructed, the sequences from the termites were dispersed within the groups of known members of parabasalids, reflecting the presence of diverse parabasalids in the hindgut of termites. There were three paraphyletic lineages of hypermastigotes represented by Pseudotrichonympha, Trichonympha, and Spirotrichonympha, in agreement with the morphology-based taxonomic groups. The analysis of the tree-root suggested that the Pseudotrichonympha group is the most probable ancient lineage of parabasalids and that the Trichonympha group is the secondly deep-branching lineage. The Spirotrichonympha group and the Trichomonadida may have emerged later.