Host range studies of GB virus-B hepatitis agent, the closest relative of hepatitis C virus, in New World monkeys and chimpanzees
Host range studies of GB virus-B hepatitis agent, the closest relative of hepatitis C virus, in New World monkeys and chimpanzees
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DOI：
10.1002/jmv.2092
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发表时间：
2001-12-01
影响因子：
12.7
通讯作者：
Purcell, RH
中科院分区：
文献类型：
--
作者：
Bukh, J;Apgar, CL;Purcell, RH
GB病毒-B（GBV-B）是黄病毒科病毒的成员。这种RNA病毒在实验感染的绢毛猴中引起急性消退性肝炎，但其天然宿主仍然未知。从含有“GB因子”的血清中回收了GBV-B和相关病毒GBV-A，据信该因子来自患有急性肝炎的外科医生（首字母缩写：GB）。GBV-B具有特殊的意义，因为它是与丙型肝炎病毒最密切相关的病毒，丙型肝炎病毒是人类急性和慢性肝病的重要原因。在本研究中，我们发现GBV-B的宿主范围包括猫头鹰猴。绢毛猴和猫头鹰猴属于新世界猴的两个不同家族。研究的两只猫头鹰猴中GBV-B的自然史与以前在绢毛猴中发现的相似，其特征是早期出现病毒血症和病毒清除。然而，在猫头鹰猴中观察到的GBV-B病毒滴度峰值（10（5）基因组当量[GE] /ml）低于在实验感染的绢毛猴中观察到的峰值（10（7）- 10（8）GE/ml），并且仅在一只动物中观察到急性肝炎。如果GBV-B确实是一种人类病毒，那么预计它会感染人类的替代品黑猩猩，因为所有公认的人类肝炎病毒都可以传播给黑猩猩并引起肝炎。然而，在目前的研究中，我们未能将GBV-B传播给一只幼稚的黑猩猩。此外，第二只未经处理的黑猩猩肝内转染了来自GBV-B感染性克隆的RNA转录物，但没有被感染。因此，黑猩猩显然不容易感染GBV-B。最后，我们未能在外科医生GB的急性期血清中检测到GBV-B。我们的数据表明GBV-B不是人类病毒，GBV-B和GBV-A一样，是新世界猴的病毒。(C)2001 Wiley-Liss，Inc.
GB virus-B (GBV-B) is a member of the Flavi-viridae family of viruses. This RNA virus causes acute resolving hepatitis in experimentally infected tamarins, but its natural host remains unknown. GBV-B and a related virus, GBV-A, were recovered from serum containing the "GB agent," which was believed to have originated from a surgeon (initials: GB) with acute hepatitis. GBV-B has special interest because it is the virus related most closely to hepatitis C virus, which is an important cause of acute and chronic liver disease in humans. In the present study, we found that the host range of GBV-B includes owl monkeys. Tamarins and owl monkeys belong to two different families of New World monkeys. The natural history of GBV-B in the two owl monkeys studied was similar to that previously found for tamarins and was characterized by early appearance of viremia and viral clearance. However, the peak viral titers of GBV-B observed in owl monkeys (10(5) genome equivalents [GE] /ml) were lower than those observed in experimentally infected tamarins (10(7) - 10(8) GE/ml) and acute hepatitis was observed in only one animal. If GBV-B were indeed a virus of humans, it would be expected to infect chimpanzees, a surrogate of humans, because all recognized human hepatitis viruses are transmissible to chimpanzees and cause hepatitis. However, in the present study, we failed to transmit GBV-B to a naive chimpanzee. In addition, a second naive chimpanzee transfected intra hepatica[ ly with RNA transcripts from an infectious clone of GBV-B did not become infected. Thus, chimpanzees are apparently not susceptible to GBV-B. Finally, we failed to detect GBV-B in acute-phase serum from surgeon GB. Our data suggest that GBV-B is not a human virus and that GBV-B, like GBV-A, is a virus of New World monkeys. (C) 2001 Wiley-Liss,Inc.