Association of genetic variants in the HDL receptor, SR-B1, with abnormal lipids in women with coronary artery disease
Association of genetic variants in the HDL receptor, SR-B1, with abnormal lipids in women with coronary artery disease
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DOI：
10.1136/jmg.40.6.453
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发表时间：
2003-06-01
影响因子：
4
通讯作者：
Topol, EJ
中科院分区：
文献类型：
--
作者：
McCarthy, JJ;Lehner, T;Topol, EJ
方法研究人群白人受试者来自GeneQuest研究，GeneQuest研究是在15个美国医学中心确定为早产儿CAD的小核心家庭的集合。每个先证者被要求在45岁(男性)或50岁(女性)之前表现出冠心病，并且至少有一个在世的同胞也符合这一标准。每个参与机构的机构审查委员会批准了该方案，所有患者都知情同意参与。在登记时，收集了临床数据和通过抽入含有EDTA的试管获得的非空腹血样。在克利夫兰诊所的中心实验室使用标准程序测量了血浆高密度脂蛋白-C、总胆固醇和甘油三酯水平。根据制造商在商业实验室的建议方案，使用Puregene试剂盒(Gentra Systems Inc)从外周血淋巴细胞中提取基因组DNA。在目前的研究中，选择了一系列无关的冠心病受试者，每个家庭只有一个人代表，优先考虑发病年龄较早的同胞。
METHODS Study populations White subjects were drawn from the GeneQuest study, a collection of small nuclear families ascertained for prematureCAD at 15 US medical centres. Each proband was required to have expressed CAD by the age of 45 if male or 50 if female, and have at least one living sib also meeting this criterion. The institutional review board at each participating institution approved the protocol, and all patients gave informed consent to participate. At enrolment, clinical data and non-fasting blood samples, obtained by drawing into tubes containing EDTA, were collected. Plasma HDL-C, total cholesterol, and TG levels were measured using standard procedures at a central laboratory at the Cleveland Clinic. Genomic DNA was isolated from peripheral blood lymphocytes using the Puregene kit (Gentra Systems Inc) according to the manufacturer’s suggested protocol at a commercial laboratory. For the purpose of the current study, a case series of unrelated subjects with CAD was selected such that only one from each family was represented, giving preference to the sib with the earlier age of onset.