Compromised vitality of spermatozoa after contact with colonic mucosa in mice: implications for fertility in colon vaginoplasty patients.
Compromised vitality of spermatozoa after contact with colonic mucosa in mice: implications for fertility in colon vaginoplasty patients.
复制标题
小鼠与结肠粘膜接触后精子活力受损：对结肠阴道成形术患者生育能力的影响。
DOI：
10.1007/s00383-018-4377-3
复制
发表时间：
2019
影响因子：
1.8
通讯作者：
Yamataka A.
中科院分区：
文献类型：
--
作者：
Kosaka S;Takeda M;Ochi T;Miyahara K;Nakamura E;Tada N;Lane GJ;Yamataka A.
研究目的结肠阴道成形术（CV）通常用于治疗泄殖腔畸形（CM）。我们用小鼠研究了与结肠粘膜接触后精子的活力作为导致不孕的一个因素。 方法将从 C57BL/6J 雄性小鼠 (n= 23) 附睾中分离的精子直接注射到雌性小鼠 (n= 45) 的阴道 (Vag 组) 或结肠 (Colo 组) 中。通过在沉积前以及沉积后 5、10、30 和 60 分钟使用计算机辅助精子分析评估活力、用 SYBR-14 和碘化丙啶染色评估活力以及通过体外受精评估生育力来确定活力。主要结果仅在 10 分钟和 60 分钟时，结肠中的活力显着下降。除 10 分钟外，结肠精子的活力在所有评估时间均显着。所有 Vag 精子均观察到正常受精，但对于 Colo，5 和 10 分钟收集的精子胚胎发育停滞，30 和 60 分钟收集的精子没有受精。 结论 精子的活力因与结肠粘膜接触而受到损害，这可能导致 CV 后 CM 不孕，因为其卵巢和输卵管 管被认为是正常的。
Aim of the studyColon vaginoplasty (CV) is often performed for cloacal malformation (CM). We used mice to study the vitality of spermatozoa after contact with colonic mucosa as a factor contributing to infertility.MethodsSpermatozoa isolated from the epididymides of C57BL/6J male mice (n= 23) were syringed directly into the vaginas (Vag-group) or colons (Colo-group) of female mice (n= 45). Vitality was determined by assessing motility using computer-assisted sperm analysis, viability by staining with SYBR-14 and propidium iodide, and fertility by in vitro fertilization, prior to deposition, and at 5, 10, 30, and 60 min after deposition.Main resultsMotility was significantly decreased in Colo only at 10 and 60 min. Viability of Colo spermatozoa was significant at all assessment times, except at 10 min. Normal fertilization was observed with all Vag spermatozoa, but with Colo, there was arrest of embryo development with spermatozoa collected at 5 and 10 min, and no fertilization with spermatozoa collected at 30 and 60 min.ConclusionsThe vitality of spermatozoa is compromised by contact with colonic mucosa which could contribute to infertility in CM after CV, because their ovaries and fallopian tubes are considered to be normal.