Comparative proteomic analysis of Aurelia coerulea for its locomotion system molecular structure-function inference
Comparative proteomic analysis of Aurelia coerulea for its locomotion system molecular structure-function inference
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Aurelia coerulea 运动系统分子结构-功能推断的比较蛋白质组学分析
DOI：
10.1016/j.jprot.2019.103509
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发表时间：
2019
影响因子：
3.3
通讯作者：
Xiao Liang
中科院分区：
文献类型：
--
作者：
Ge Mengxiao;Liu Wenwen;Ma Chaoqun;Yan Zhenpeng;Liang Hongyu;Xu Zheng;Mariottini Gian Luigi;Zhang Jing;Zhao Xianxian;Yang Yi;Xiao Liang
水母的节律性收缩和自主运动在捕食、生产和迁移过程中起着关键作用。方法采用液相色谱-质谱联用/质谱联用（LC-MS/MS）技术，对蓝齿水母（harelia coerulea）四个独立的身体部位（Bell、Tentacle、Oral arm和Gastric pouch）进行蛋白质组学比较。结果共鉴定出分子量在10.6 ~ 980.9 kDa范围内的13429个肽段和1916个蛋白，其中在胃囊、触角、口臂和贝尔中分别匹配了1916、1562、1474和1441个蛋白。基因本体论（GO）分析表明，翻译、胞浆和ATP结合占据了生物过程、细胞成分和分子功能三个子域的最高差异术语。共绘制了326条通路，主要与细胞内合成、代谢和细胞内功能相关。此外，共鉴定出27种收缩机械相关蛋白，包括22种肌凝蛋白，3种肌动蛋白和2种原肌凝蛋白。结论sour的结果提供了水母四个独立身体部位的组成谱。其中的肌肉蛋白的鉴定将有助于了解水母运动系统中的结构和功能关系，以及它们的运作机制。组学研究对双侧和径向对称动物运动系统发育过程中基因和蛋白质网络的功能有了新的全面认识。采用无标记的纳米lc -MS/MS方法对月亮水母的四个独立身体部位进行了蛋白质组学比较。胼胝体，包括脐、触手、口臂和胃袋。除了传统的生物信息学分析（如GO和KEGG）外，我们还扫描了运动相关成分，排列了它们的序列，模拟了三维结构，并进行了分子系统发育分析。我们的研究提供了水母四个独立身体部位的组成概况。其中的肌肉蛋白的鉴定将有助于了解水母运动系统中的结构和功能关系，以及它们的运作机制。
BackgroundRhythmic contraction and autonomous movement play a key role in the predation, production and displacement of jellyfish.MethodsFour independent body parts of the jellyfishAurelia coerulea, including Bell, Tentacle, Oral arm and Gastric pouch were extracted and have been carried out a compared proteomics by liquid chromatography-mass spectrometry/mass-spectrometry (LC-MS/MS).ResultsA total of 13,429 peptides and 1916 proteins with molecular weights in the range of 10.6–980.9 kDa were identified, where 1916, 1562, 1474 and 1441 proteins were matched in the Gastric pouch, Tentacle, Oral arm and Bell, respectively. Gene Ontology (GO) analysis showed that translation, cytoplasma and ATP binding occupy the top differential terms of the three subdomains Biological process, Cellular Component and Molecular Function. A total of 326 pathways were successfully mapped that are mainly associated with intracellular synthesis, metabolism as well as intracellular functions. Moreover, a total of 27 contractile machinery associated proteins including 22 myosin, 3 actin and 2 tropomyosin were identified.ConclusionsOur results provide a composition profile in the four independent body parts of the jellyfishA. coerulea, of which the identified muscular proteins will greatly help in the understanding of the structural and functional relationship, as well as their operating mechanisms in the jellyfish locomotion system.SignificanceOmics studies have gained a new overall insight into the function of gene and protein networks during the development of motor systems in both bilateral and radial symmetrical animals. A compared proteomics using the label-free method of nano-LC-MS/MS has been performed through the four independent body parts of the moon jellyfishA. coerulea, including Bell, Tentacle, Oral arm and Gastric pouch. In addition to conventional bioinformatics analyses such as GO and KEGG, we have scanned the locomotion-related components, aligned their sequences, simulated three dimensional structures as well as did the molecular phylogenetic analyses. Our investigation provides a composition profile in the four independent body parts of the jellyfishA. coerulea, of which the identified muscular proteins will greatly help in the understanding of the structural and functional relationship, as well as their operating mechanisms in the jellyfish locomotion system.