High-content screening of peptide-based non-viral gene delivery systems
High-content screening of peptide-based non-viral gene delivery systems
复制标题
DOI：
10.1016/j.jconrel.2011.09.078
复制
发表时间：
2012-03-28
影响因子：
10.8
通讯作者：
Mastrobattista, Enrico
中科院分区：
文献类型：
--
作者：
de Raad, Markus;Teunissen, Erik A.;Mastrobattista, Enrico
High-content screening (HCS) uses high-capacity automated fluorescence imaging for the quantitative analysis of single cells and cell populations. Here, we developed an HCS assay for rapid screening of non-viral gene delivery systems as exemplified by the screening of a small library of peptide-based transfectants. These peptides were simultaneously screened for transfection efficiency, cytotoxicity, induction of cell permeability and the capacity to transfect non-dividing cells. We demonstrated that HCS is a valuable extension to the already existing screening methods for the in vitro evaluation of non-viral gene delivery systems with the added value that multiple parameters can be screened in parallel thereby obtaining more information from a single screening event, which will accelerate the development of novel gene delivery systems. (C) 2011 Published by Elsevier B. V.
