Endurance training in humans leads to fiber type-specific increases in levels of peroxisome proliferator-activated receptor-γ coactivator-1 and peroxisome proliferator-activated receptor-α in skeletal muscle
Endurance training in humans leads to fiber type-specific increases in levels of peroxisome proliferator-activated receptor-γ coactivator-1 and peroxisome proliferator-activated receptor-α in skeletal muscle
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DOI：
10.2337/diabetes.52.12.2874
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发表时间：
2003-12-01
期刊：
影响因子：
7.7
通讯作者：
Dériaz, O
中科院分区：
文献类型：
--
作者：
Russell, AP;Feilchenfeldt, J;Dériaz, O
过氧化物酶体增殖物激活受体（PPAR）-γ共激活因子-1（PGC-1）可诱导线粒体生物合成，并与氧化型I型肌纤维的发育有关。PPAR亚型α、β/δ和γ控制参与脂肪酸和葡萄糖代谢的基因的转录。由于耐力训练增加骨骼肌线粒体和I型纤维含量和脂肪酸氧化能力，我们的目的是确定这些增加是否可以通过对PGC-1或PPAR-alpha，-beta/delta和-gamma的可能影响来介导。7名健康男性进行了6周的耐力训练，并在其股外侧肌活检中测量了PGG-1和PPA-R-α、-β/δ和-γ mRNA的表达水平以及PGC-1和PPAR-alpha蛋白的纤维类型分布。耐力训练后PGC-1和PPAR-a mRNA表达分别增加2.7倍和2.2倍（P < 0.01）。PGC-1的表达是2.2-和6倍以上的IIa型比I型和IIx纤维，分别。耐力训练后，IIa型肌纤维增加2.8倍，I、IIx型肌纤维增加1.5倍（P < 0.015）。PPAR-alpha在I型肌纤维中的表达是II型肌纤维的1.9倍，在耐力训练后分别增加了3.0倍和1.5倍（P < 0.001）。本研究中报告的PGC-1和PPAR-alpha水平的增加可能在已知由耐力训练诱导的肌肉线粒体含量、氧化表型和胰岛素敏感性的变化中起重要作用。
The peroxisome proliferator-activated receptor (PPAR)-gamma coactivator-1 (PGC-1) can induce mitochondria biogenesis and has been implicated in the development of oxidative type I muscle fibers. The PPAR isoforms alpha, beta/delta, and gamma control the transcription of genes involved in fatty acid and glucose metabolism. As endurance training increases skeletal muscle mitochondria and type I fiber content and fatty acid oxidative capacity, our aim was to determine whether these increases could be mediated by possible effects on PGC-1 or PPAR-alpha, -beta/delta, and -gamma. Seven healthy men performed 6 weeks of endurance training and the expression levels of PGG-1 and PPA-R-alpha, -beta/delta, and -gamma mRNA as well as the fiber type distribution of the PGC-1 and PPAR-alpha proteins were measured in biopsies from their vastus lateralis muscle. PGC-1 and PPAR-a mRNA expression increased by 2.7- and 2.2-fold (P < 0.01), respectively, after endurance training. PGC-1 expression was 2.2- and 6-fold greater in the type IIa than in the type I and IIx fibers, respectively. It increased by 2.8-fold in the type IIa fibers and by 1.5-fold in both the type I and IIx fibers after endurance training (P < 0.015). PPAR-alpha was 1.9-fold greater in type I than in the II fibers and increased by 3.0-fold and 1.5-fold in these respective fibers after endurance training (P < 0.001). The increases in PGC-1 and PPAR-alpha levels reported in this study may play an important role in the changes in muscle mitochondria content, oxidative phenotype, and sensitivity to insulin known to be induced by endurance training.