Molecular and functional analysis of cellular phenomena using single-cell mass spectrometry.
Molecular and functional analysis of cellular phenomena using single-cell mass spectrometry.
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DOI：
10.1248/bpb.b212012
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发表时间：
2012
影响因子：
2
通讯作者：
N. Tsuyama;H. Mizuno;T. Masujima
中科院分区：
文献类型：
--
作者：
N. Tsuyama;H. Mizuno;T. Masujima
单细胞分析作为研究细胞和分子行为精确机制的工具，在生物学研究的许多领域引起了人们的关注。灵敏的质谱技术的发展使人们能够研究单个细胞或小区域中的分子。基质辅助激光解吸/电离成像质谱法和二次离子质谱法使用样品板上的样品的原位电离来将分子分布可视化为来自质谱的图像。已经开发了几种最初回收单个细胞然后电离的单细胞质谱技术。其中，只有称为活单细胞质谱的纳米喷雾介导的采样和电离可以用于实时分析。本文详细介绍了这种方法。
Single-cell analysis has attracted attention in many fields of biological studies as a tool to survey the precise mechanisms of cellular and molecular behavior. The development of sensitive mass spectrometry allows the study of molecules in single cells or small regions. Matrix-assisted laser desorption/ionization imaging mass spectrometry and secondary-ion mass spectrometry use in situ ionization of specimens on sample plates to visualize molecular distributions as images from mass spectra. Several single-cell mass spectrometry technologies that initially recover a single cell followed by ionization have been developed. Among them, only nanospray-mediated sampling and ionization named Live Single-cell Mass Spectrometry can be used for real-time analysis. This paper explains that method in detail.