Influence of late N‐acetylcysteine administration on cardiac remodeling in long‐term follow‐up postinfarction rats (862.3)
Influence of late N‐acetylcysteine administration on cardiac remodeling in long‐term follow‐up postinfarction rats (862.3)
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DOI：
10.1096/fasebj.28.1_supplement.862.3
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发表时间：
2014-04
期刊：
影响因子：
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通讯作者：
V. Lopes;S. O. Oliveira Júnior;M. Cezar;R. Damatto;C. Bonomo;L. Pagan;A. Lima;M. Gomes;L. Zornoff;K. Okoshi;M. Okoshi;P. Martinez
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文献类型：
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作者：
V. Lopes;S. O. Oliveira Júnior;M. Cezar;R. Damatto;C. Bonomo;L. Pagan;A. Lima;M. Gomes;L. Zornoff;K. Okoshi;M. Okoshi;P. Martinez
本研究旨在评价晚期N-乙酰半胱氨酸（NAC）给药对长期随访梗死后大鼠心脏重塑的影响。方法：采用左冠状动脉阻断法制备心肌梗死模型。4个月后，将大鼠分为3组：假手术组（n=13）、MI-C组（MI未治疗，n=16）和MI-NAC组（MI用N-乙酰半胱氨酸治疗，120 mg/kg/天，n=16）。术后6个月，经胸多普勒超声心动图评价心脏结构和功能，次日处死大鼠。通过组织学分析测量脑梗死的大小，并且具有小MI的大鼠（0.05; Student’s t检验）。MI‐C和MI‐NAC之间的右心室（RV）和肺重量、RV/体重（BW）和肺/BW比值相似，且高于假手术组。MI‐C组的左心室舒张期内径、左心房（LA）内径和LA/BW比值高于假手术组，MI ‐ C组与MI‐NAC组相似。MI‐C和MI‐NAC之间的收缩功能变量面积缩短分数和后壁缩短速度相似，均低于Sham。关于舒张功能，MI-C中的E波/A波比值高于Sham和MI-NAC（Sham：1.47±0.14; MI-C：4.83±2.41; MI-NAC：3.25±1.85; p<0.001，ANOVA和Bonferroni）。结论：晚期N-乙酰半胱氨酸治疗减轻了长期随访的梗死后大鼠的舒张功能障碍。
This study aimed to evaluate the influence of late N‐acetylcysteine (NAC) administration on cardiac remodeling in long‐term follow‐up postinfarction rats. Methods: Myocardial infarction (MI) was induced by left coronary occlusion. Four months later, rats were assigned to three groups: Sham (n=13), MI‐C (MI without treatment, n=16), and MI‐NAC (MI treated with N‐acetylcysteine, 120 mg/kg/day, n=16). Six months after surgery, cardiac structures and function were evaluated by transthoracic Doppler echocardiogram and rats were euthanized the next day. Infarct size was measured by histological analysis, and rats with small MI (0.05; Student’s t test). Right ventricle (RV) and lung weight, RV/body weight (BW) and lung/BW ratios were similar between MI‐C and MI‐NAC and higher than Sham. Left ventricle diastolic diameter, left atrium (LA) diameter, and LA/BW ratio were higher in MI‐C than Sham and similar between MI‐C and MI‐NAC. The systolic function variables fractional area shortening and posterior wall shortening velocity were similar between MI‐C and MI‐NAC and both lower than Sham. Concerning diastolic function, E wave‐to‐A wave ratio was higher in MI‐C than in both Sham and MI‐NAC (Sham: 1.47±0.14; MI‐C: 4.83±2.41; MI‐NAC: 3.25±1.85; p<0.001, ANOVA and Bonferroni). Conclusion: Late N‐acetylcysteine treatment attenuates diastolic dysfunction in long‐term follow‐up postinfarction rats.