DEPLETION OF TOTAL SALIVARY-GLAND PROTEIN IN BLOOD-FED ANOPHELES MOSQUITOS
DEPLETION OF TOTAL SALIVARY-GLAND PROTEIN IN BLOOD-FED ANOPHELES MOSQUITOS
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DOI：
10.1093/jmedent/32.3.300
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发表时间：
1995-05-01
影响因子：
2.1
通讯作者：
SEELEY, DC
中科院分区：
文献类型：
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作者：
GOLENDA, CF;KLEIN, T;SEELEY, DC
人疟疾四种疟蚊载体中唾液腺总蛋白的减少，斯氏按蚊阿尔布曼纳斯·魏德曼，安。gambiae Giles和An。freeborni Aitken，在蚊子将血液喂饱人类志愿者、仓鼠或通过Baudruche人工膜后进行定量。6只未喂蚊池唾液腺蛋白含量变化范围为4.33 ~ 7.91 μ g/ml。6个池型唾液腺总蛋白差异在1.77 ~ 3.12 （mu g/ml）之间。吸血至饱腹的蚊子唾液腺总蛋白显著低于未吸血的对照组。An唾液腺总蛋白的减少。在人类志愿者、仓鼠和Baudruche膜上充血的freeborni和stephensi血液分别为24% ~ 46%、43% ~ 56%和24% ~ 51%。让斯氏蚊在0（未喂食）、0.5、1.0、2.0 min或饱食（> ~ 5 min）时间内吸血。随着摄食时间的延长，唾液腺蛋白质总量显著降低。随着时间的推移，这种减少是成比例的，这表明从开始（探查）吸血到蚊子口器充血时撤回唾液持续发生。这些数据表明，在吸血过程中，不同物种之间的唾液腺输出量（以唾液腺中蛋白质的消耗量为衡量标准）没有明显差异，而且在蚊子进食到饱的过程中，唾液腺中蛋白质的消耗会持续发生。
Reduction in total salivary gland protein from four anopheline vectors of human malaria, Anopheles stephensi Listen, An. albimanus Wiedmann, An. gambiae Giles, and An. freeborni Aitken, was quantified after mosquitoes blood-fed to repletion on human volunteers, hamsters or through a Baudruche artificial membrane. Total salivary gland protein from pools of six unfed mosquitoes ranged from 4.33 to 7.91 mu g/ml. The difference between the total protein of glands from unfed and blood-fed mosquitoes for all species ranged from 1.77 to 3.12 (mu g/ml for six pooled salivary glands. Total salivary gland protein for mosquitoes blood-fed to repletion was significantly less than that of unfed controls from the same cohort. Reduction in total salivary gland protein for An. freeborni and An stephensi blood fed to repletion on human volunteers, hamsters, and a Baudruche membrane ranged from 24 to 46%, from 43 to 56%, and from 24 to 51%, respectively An. stephensi mosquitoes were allowed to blood feed on humans for 0 (unfed), 0.5-, 1.0-, 2.0-min time periods or to repletion (>2-5 min). As feeding time increased, there was a significant decrease in total amount of protein in the salivary glands. This decrease was proportional over time, indicating that salivation occurred continuously from the beginning (probing) of blood feeding to withdrawal of the mosquito mouthparts at repletion. These data indicate that during blood feeding there was no apparent difference between species in the salivary gland output measured as amount of protein depleted from the salivary glands and that depletion of salivary protein from the glands occurred continuously as mosquitoes fed to repletion.