Effects of cellular viscoelasticity in lifetime extraction of single receptor-ligand bonds.
Effects of cellular viscoelasticity in lifetime extraction of single receptor-ligand bonds.
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DOI：
10.1103/physreve.91.062701
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发表时间：
2015-06
期刊：
影响因子：
--
通讯作者：
V. Gupta
中科院分区：
文献类型：
--
作者：
V. Gupta
单分子力谱法被广泛用于通过分析通过向附着于分子间键的细胞、胶囊和珠施加机械力获得的键断裂数据来确定解离的动力学参数。键断裂数据是在恒定力或恒定加载速率下的实验中获得的。我们探讨了细胞粘弹性在恒定力实验中的作用。具体而言，我们进行Monte Carlo模拟的键断裂在一个给定的恒定的力，以获得作为力的函数的键寿命的存在下，由于细胞粘弹性的键力调制，探索其对键寿命的影响。
Single-molecule force spectroscopy is widely used to determine kinetic parameters of dissociation by analyzing bond rupture data obtained via applying mechanical force to cells, capsules, and beads that are attached to an intermolecular bond. The bond rupture data are obtained in experiments either at a constant force or at a constant loading rate. We explore the effect of cellular viscoelasticity in constant-force experiments. Specifically, we perform Monte Carlo simulations of bond rupture at a given constant force to obtain the bond lifetime as a function of force in the absence and in the presence of bond force modulation due to cellular viscoelasticity, to explore its effect on the bond lifetime.