Structure-dependent nonenzymatic deamidation of glutaminyl and asparaginyl pentapeptides
Structure-dependent nonenzymatic deamidation of glutaminyl and asparaginyl pentapeptides
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DOI：
10.1111/j.1399-3011.2004.00151.x
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发表时间：
2004-05-01
期刊：
影响因子：
--
通讯作者：
Robinson, AB
中科院分区：
文献类型：
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作者：
Robinson, NE;Robinson, ZW;Robinson, AB
在pH为7.4，温度为37.0℃时，52个谷氨酰基和52个天冬酰胺基五肽的非酶脱酰胺速率。用直接进样质谱法测定了0.15M Tris-HCl缓冲液。这些和先前报道的306个天冬氨酸氨基被结合在一个自洽的多肽脱酰胺模型中。该模型定量依赖于多肽结构，涉及丁二酰亚胺、戊二酰亚胺和水解机理。将实验值和合适的插值值相结合，得到了pH为7.4、温度为37.0℃的脱酰胺速率值。0.15M Tris-HCl缓冲液，用于GlyXxxAsnYyyGly和GlyXxxGlnYyyGly中普通氨基酸残基的全套648个单酰胺排列。因此，关于多肽中依赖序列的脱酰胺化的知识被扩展到包括在2-50年范围内非常长的脱酰胺化一半时间。
Nonenzymatic deamidation rates for 52 glutaminyl and 52 asparaginyl pentapeptides in pH 7.4, 37.0 degreesC. 0.15 M Tris-HCl buffer have been determined by direct injection mass spectrometry. These and the previously reported 306 asparginyl rates have been combined in a self-consistent model for peptide deamidation. This model depends quantitatively upon peptide structure and involves succinimide, glutarimide and hydrolysis mechanisms. The experimental values and suitable interpolated values have been combined to provide deamidation rate values in pH 7.4, 37.0 degreesC. 0.15 M Tris-HCl buffer for the entire set of 648 single-amide permutations of ordinary amino acid residues in GlyXxxAsnYyyGly and GlyXxxGlnYyyGly. Thus, knowledge about sequence-dependent deamidation in peptides is extended to include very long deamidation half-times in the range of 2-50 years.