The cytoplasmic expression of CD3 antigens in normal and malignant cells of the T lymphoid lineage.
The cytoplasmic expression of CD3 antigens in normal and malignant cells of the T lymphoid lineage.
复制标题
DOI：
10.4049/jimmunol.138.2.648
复制
发表时间：
1987-01
影响因子：
4.4
通讯作者：
D. Campana;J. Thompson;P. Amlot;S. Brown;G. Janossy
中科院分区：
文献类型：
--
作者：
D. Campana;J. Thompson;P. Amlot;S. Brown;G. Janossy
在人和选定灵长类动物的骨髓、胸腺和血液中，检测与不同比例胸腺细胞（抗CD 3a：UCHT 1、抗CD 3b：T10 B 9和抗CD 3c：OKT 3）反应的抗CD 3（T3）Ab的细胞质（cCD 3）和膜（mCD 3）表达。cCD 3a和cCD 3c在大的、BrdU掺入的、强TdT+胸腺母细胞的核周和高尔基体区域中的表达可能代表T细胞定型的最早迹象之一，因为这些母细胞是CD 1-、CD 4-、CD 8-和mCD 3-。cCD 3+、TdT+细胞通常局限于胸腺，在骨髓的TdT+细胞中不存在。使用的抗CD 3b Ab T10 B 9与其他抗CD 3 Ab共加帽和共调节，是膜水平的T细胞特异性试剂，但不结合核周cCD 3。相反，该试剂与人类和灵长类动物S中非T细胞中的丝状细胞质网络交叉反应。oedipus和M.尽管它们的T细胞对mCD 3呈阴性。研究的所有T-ALL病例的特征：cCD 3+、CD 7+沿着核TdT+，提示对早期胸腺母细胞的谱系保真度。作为一个明显的对比，cCD 3是缺乏常见的ALL和AML，包括同时表达CD 7和髓系抗原的情况下。因此，cCD 3、TdT组合为正常胸腺外的残留T-ALL原始细胞提供了非常灵敏的测定。
Anti-CD3 (T3) Ab reacting with different proportions of thymocytes (anti-CD3a: UCHT1, anti-CD3b: T10B9, and anti-CD3c: OKT3) were tested for cytoplasmic (cCD3) and membrane (mCD3) expression in the bone marrow, thymus, and blood in man and selected primates. The expression of cCD3a and cCD3c in the perinuclear and Golgi area of large, BrdU-incorporating, strongly TdT+ thymic blasts probably represents one of the earliest signs of T cell commitment, because these blast cells are CD1-, CD4-, CD8-, and mCD3-. The cCD3+, TdT+ cells are normally restricted to the thymus and are absent among the TdT+ cells of bone marrow. The anti-CD3b Ab used, T10B9, co-caps and co-modulates with the other anti-CD3 Ab and is a T cell-specific reagent at a membrane level but does not bind to perinuclear cCD3. Instead, this reagent cross-reacts with a filamentous cytoplasmic network in non-T cells in man and in primates S. oedipus and M. rhesus despite their T cell negativity for mCD3. The characteristics of all T-ALL cases studied: cCD3+, CD7+ along with nuclear TdT+ suggest lineage fidelity to early thymic blasts. As a marked contrast, cCD3 is absent in common ALL and in AML, including cases that concomitantly express CD7 and myeloid antigens. Thus, the cCD3, TdT combination provides a very sensitive assay for residual T-ALL blasts outside the normal thymus.