Fluorescence quenching studies of nitrated polycyclic aromatic hydrocarbons
Fluorescence quenching studies of nitrated polycyclic aromatic hydrocarbons
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DOI：
10.1002/bio.1205
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发表时间：
2011-03-01
期刊：
影响因子：
2.9
通讯作者：
Miller, James N.
中科院分区：
文献类型：
--
作者：
Al-Kindy, Salma M. Z.;Miller, James N.
硝化多环芳烃（NPAHs）广泛分布于环境中，具有致突变性和潜在的致癌性，因此对其进行分析具有重要意义。已知芳香化合物中的亚硝基取代基猝灭荧光，而NPAHs没有固有荧光，但可以通过它们对其他荧光团的猝灭作用来确定它们。研究了几种重要的NPAHs对1,2,3,4-四氢-1-萘酚、5,6,7,8-四氢-1-萘酚、4-（2-羟基-4-磺基-1-萘偶氮）-2-萘羧酸和7-氨基-4-甲基香豆素的猝灭作用。这些荧光团的单线态发射被所有的NPAHs有效地猝灭，猝灭遵循斯特恩-沃尔默关系。在每种情况下，淬灭常数和检测限以及淬灭器的线性范围已经确定：检测限约为1 μ M. Copyright (C) 2010 John Wiley & Sons, Ltd。
The analysis of nitrated polycyclic aromatic hydrocarbons (NPAHs) is of great importance because of the mutagenicity and possible carcinogenic activity of these compounds, which are distributed widely in the environment. Nitrosubstituents in aromatic compounds are known to quench fluorescence and NPAHs have no intrinsic fluorescence, but they can be determined using their quenching effects on other fluorophores. The quenching effects of several important NPAHs on 1,2,3,4-tetrahydro-1-naphthol,5,6,7,8-tetrahydro-1-naphthol,4-(2-hydroxy-4-sulfo-1-naphthylazo)-2-naphthalene carboxylic acid and 7-amino-4-methyl coumarin have been studied. The singlet emission of these fluorophores is efficiently quenched by all the NPAHs, the quenching following the Stern-Volmer relationship. Quenching constants and the limits of detection and linear ranges of the quenchers have been determined in each case: the limits of detection are ca 1 mu M. Copyright (C) 2010 John Wiley & Sons, Ltd.