Delaminated Layered Double Hydroxide Nanosheets as an Efficient Vector for DNA Delivery
Delaminated Layered Double Hydroxide Nanosheets as an Efficient Vector for DNA Delivery
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分层层状双氢氧化物纳米片作为 DNA 传递的有效载体
DOI：
10.1166/jbn.2016.2252
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发表时间：
2016-05-01
影响因子：
2.9
通讯作者：
Wan, Yinglang
中科院分区：
文献类型：
--
作者：
Wang, Junya;Bao, Wenlong;Wan, Yinglang
研究了分层的Mg-Al-lactate和Mg-Al-acetate层状双氢氧化物（LDHs）纳米片（Mg-Al-lactate-NS、Mg-Al-acetate-NS）作为DNA吸附和递送至293 T细胞的有效载体的性能。采用XRD、SEM、TEM和Zeta电位等手段对乳酸镁铝和醋酸镁铝水滑石进行了表征。通过改变吸附剂的用量和吸附时间来评价乳酸镁铝盐和醋酸镁铝盐对鲑鱼精DNA的吸附能力，结果表明乳酸镁铝盐对鲑鱼精DNA的吸附能力（649.6 μ g mg（-1））远高于醋酸镁铝盐（340.0 μ g mg（-1））。XRD分析表明，Mg-Al-Lactatate-NS-DNA生物无机纳米杂化材料在吸附DNA后仍保持剥离状态。由于Mg-Al-Lactate-NS-DNA很难从溶液中分离出来，因此还采用电泳分析法检测吸附后溶液中的游离DNA。使用293 T细胞的细胞毒性研究证实，Mg-Al-乳酸盐-NS的毒性低于Mg-Al-乙酸盐-NS，因为需要更小剂量的这种LDH来将相同量的鲑鱼DNA递送至293 T细胞。细胞摄取和共聚焦成像研究表明，Mg-Al-lactate-NS成功转染ssDNA-FITC到293 T细胞中。然而，FITC偶联的单链DNA未能内化到这些细胞中。乳酸镁铝水滑石纳米片具有良好的DNA吸附和递送能力，是一种比体相LDH更好的DNA载体。
The performance of delaminated Mg-Al-lactate and Mg-Al-acetate layered double hydroxides (LDHs) nanosheets (Mg-Al-lactate-NS, Mg-Al-acetate-NS) as efficient vectors for DNA adsorption and delivery to 293T cells was investigated. Mg-Al-lactate and Mg-Al-acetate LDHs were delaminated into single layers in water and were characterized using XRD, SEM, TEM, and Zeta potential measurements. The salmon sperm DNA adsorption capacity of Mg-Al-lactate-NS and Mg-Al-acetate-NS were evaluated by varying the adsorbent dosage and contacting time, which suggested that Mg-Al-lactate-NS had much higher adsorption capacity (649.6 mu g mg(-1)) than that of Mg-Al-acetate-NS (340.0 mu g mg(-1)). XRD analysis indicated that after DNA adsorption the Mg-Al-lactate-NS-DNA bio-inorganic nanohybrid still stayed in an exfoliated form. Due to the difficulty in separating the Mg-Al-lactate-NS-DNA from solution, electrophoresis analysis was also applied to detect the free DNA in solution after adsorption. Cytotoxicity studies using 293T cells verified that Mg-Al-lactate-NS was less toxic than Mg-Al-acetate-NS as a smaller dose of this LDH was needed to deliver the same amount of salmon DNA to 293T cells. Cellular uptake and confocal imaging studies demonstrated that Mg-Al-lactate-NS was successful in transfection of ssDNA-FITC into 293T cells. However, the FITC-coupled single strand DNA was failed to be internalized into these cells. The excellent DNA adsorption and delivery capacities indicate that delaminated Mg-Al-lactate LDHs nanosheets are a better DNA vector than bulk phase LDH.