Acidic-pH-Activatable Fluorescence Probes for Visualizing Exocytosis Dynamics
Acidic-pH-Activatable Fluorescence Probes for Visualizing Exocytosis Dynamics
复制标题
DOI：
10.1002/anie.201402030
复制
发表时间：
2014-06-01
影响因子：
16.6
通讯作者：
Hirose, Kenzo
中科院分区：
文献类型：
--
作者：
Asanuma, Daisuke;Takaoka, Yousuke;Hirose, Kenzo
胞吐动力学的实时成像对于精确时空理解分泌现象至关重要，但目前的方法存在严重的局限性。我们设计并合成了小分子荧光探针，这些探针经过化学优化，可检测酸性囊泡内 pH 值，并确定它们可用于灵敏、可靠地可视化刺激后的囊泡动力学。这种以高时空精度可视化胞吐作用以及内吞作用/再酸化过程的简单技术有望成为研究涉及 pH 值变化的动态细胞现象的有力工具。
Live imaging of exocytosis dynamics is crucial for a precise spatiotemporal understanding of secretion phenomena, but current approaches have serious limitations. We designed and synthesized small-molecular fluorescent probes that were chemically optimized for sensing acidic intravesicular pH values, and established that they can be used to sensitively and reliably visualize vesicular dynamics following stimulation. This straightforward technique for the visualization of exocytosis as well as endocytosis/reacidification processes with high spatiotemporal precision is expected to be a powerful tool for investigating dynamic cellular phenomena involving changes in the pH value.