Quality evaluation of the artemisinin-producing plant Artemisia annua L. based on simultaneous quantification of artemisinin and six synergistic components and hierarchical cluster analysis
Quality evaluation of the artemisinin-producing plant Artemisia annua L. based on simultaneous quantification of artemisinin and six synergistic components and hierarchical cluster analysis
复制标题
基于青蒿素和六种协同成分同时定量和层次聚类分析的青蒿素生产植物青蒿质量评价
DOI：
10.1016/j.indcrop.2018.03.043
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发表时间：
2018-08-01
影响因子：
5.9
通讯作者：
Wang, Manyuan
中科院分区：
文献类型：
--
作者：
Qiu, Feng;Wu, Sha;Wang, Manyuan
[医]黄花蒿（A.黄花蒿是应用最广泛的传统中药之一，也是青蒿素工业化生产的唯一天然来源。A.含有多种倍半萜、黄酮和香豆素类化合物。黄花蒿可增强青蒿素的药理作用。但不同产地、不同部位的黄芪中这些成分的含量差异较大。annua。为评价和鉴别青蒿素的质量，建立了同时测定青蒿素及其6种增效成分的高效液相色谱-串联质谱（HPLC-MS/MS）分析方法。annua。分析一个样品的时间仅为6.0 min，该方法可作为一种快速、准确的质量评价方法。一年生植物来自不同的原产地和植物的不同部分。聚类分析结果表明，东莨菪内酯和/或青蒿素及6种增效成分的含量可作为区分不同采收期和不同部位青蒿的指标。annua。
Artemisia annua L. (A. annua) is one of the most widely used traditional Chinese Medicine, and is also an exclusive natural origin of artemisinin for the industrial production. Several sesquiterpenes, flavonoids and coumarin contained in A. annua could enhance the pharmacological effects of artemisinin. However, the contents of these components vary significantly in different places of origin and different parts of A. annua. To evaluate and differentiate its quality, an accurate and rapid high-performance liquid chromatography tandem mass spectrometric (HPLC-MS/MS) assay has been developed and validated for the simultaneous determination of artemisinin and six synergistic components in A. annua. The run time of analyzing one sample is only 6.0 min. The established method could serve as an accurate and rapid assay for the quality evaluation of A. annua from different places of origin and different parts of the plant. The hierarchical cluster analysis results indicated that the content of scopoletin and/or all seven components (artemisinin and six synergistic components) might be utilized to differentiate different parts and different plucking times of A. annua.