Systemic versus tissue-level prolactin signaling in a teleost during a tidal cycle
Systemic versus tissue-level prolactin signaling in a teleost during a tidal cycle
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潮汐周期期间硬骨鱼的全身催乳素信号与组织水平的比较
DOI：
10.1007/s00360-019-01233-9
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Lerner, Darren T.
中科院分区：
文献类型：
--
作者：
Seale, Andre P.;Pavlosky, K. Keano;Celino-Brady, Fritzie T.;Yamaguchi, Yoko;Breves, Jason P.;Lerner, Darren T.
泛盐莫桑比克罗非鱼（Oreochromis mossambicus）原产于河口，在那里它们遇到环境盐度的潮汐波动。这些波动可能是剧烈的，使个体在一个潮汐周期内受到淡水（FW < 0.5‰）和海水（SW 35‰）的盐度特征的影响。在本研究中，我们在模拟罗非鱼原生栖息地动态条件的潮汐条件下饲养罗非鱼。罗非鱼在24小时内每隔3小时取样一次，以暂时确定催乳素（PRL）信号是如何与编码渗透调节分支效应的基因并行调节的。测定血浆渗透压、血浆prl177和prl188浓度、垂体prl177和prl188基因表达、支气管prl受体（prlr1和prlr2）、Na+/Cl−共转运体（ncc2）、Na+/K+/2Cl−共转运体（nkcc1a）、Na+/K+- atp酶（nkaα 1a和nkaα1b）、囊性纤维化跨膜调节剂（cftr）和水通道蛋白3（aqp3）基因表达。在24小时的采样期间，血浆渗透压反映了罗非鱼是在潮汐周期的FW期还是SW期采样，而垂体泌素表达和血浆PRL水平保持稳定。ncc2、nkcc1a、nkaα1a、nkaα1b、cftr和aqp3基因的鳃区表达模式表明，暴露在潮汐变化的盐度下的鱼与处于静态SW条件下的鱼以类似的方式调节这些基因转录物的表达。相比之下，在整个潮汐循环中，鳃prlr1和prlr2水平高度不稳定。我们得出结论，内分泌信号的局部（鳃）调节是泛盐鱼类（如莫桑比克罗非鱼）在动态盐度条件下茁壮成长的基础。
Euryhaline Mozambique tilapia (Oreochromis mossambicus) are native to estuaries where they encounter tidal fluctuations in environmental salinity. These fluctuations can be dramatic, subjecting individuals to salinities characteristic of fresh water (FW < 0.5‰) and seawater (SW 35‰) within a single tidal cycle. In the current study, we reared tilapia under a tidal regimen that simulated the dynamic conditions of their native habitat. Tilapia were sampled every 3 h over a 24 h period to temporally resolve how prolactin (PRL) signaling is modulated in parallel with genes encoding branchial effectors of osmoregulation. The following parameters were measured: plasma osmolality, plasma PRL177and PRL188concentrations, pituitaryprl177andprl188gene expression, and branchialprl receptor(prlr1andprlr2),Na+/Cl−-cotransporter(ncc2),Na+/K+/2Cl−-cotransporter(nkcc1a),Na+/K+-ATPase(nkaα1aandnkaα1b),cystic fibrosis transmembrane regulator(cftr), andaquaporin 3(aqp3) gene expression. Throughout the 24 h sampling period, plasma osmolality reflected whether tilapia were sampled during the FW or SW phases of the tidal cycle, whereas pituitaryprlgene expression and plasma PRL levels remained stable. Branchial patterns ofncc2, nkcc1a, nkaα1a,nkaα1b, cftr, andaqp3gene expression indicated that fish exposed to tidally changing salinities regulate the expression of these gene transcripts in a similar fashion as fish held under static SW conditions. By contrast, branchialprlr1andprlr2levels were highly labile throughout the tidal cycle. We conclude that local (branchial) regulation of endocrine signaling underlies the capacity of euryhaline fishes, such as Mozambique tilapia, to thrive under dynamic salinity conditions.
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影响因子：
2.7
作者：
A. Seale;J. C. Fiess;Tetsuya Hirano;Ian M. Cooke;E. Grau
通讯作者：
A. Seale;J. C. Fiess;Tetsuya Hirano;Ian M. Cooke;E. Grau
渗透压和生长抑素对硬骨鱼罗非鱼（Oreochromis mossambicus）渗透敏感催乳素细胞 cAMP 信使系统的影响。
影响因子：
2.7
作者：
L. Helms;E. Grau;R. Borski
通讯作者：
R. Borski
影响因子：
--
作者：
BORSKI, RJ;HANSEN, MU;GRAU, EG
通讯作者：
GRAU, EG
影响因子：
2.7
作者：
T. Wigham;J. Ball
通讯作者：
T. Wigham;J. Ball
影响因子：
2.8
作者：
S. Watanabe;T. Kaneko;K. Aida
通讯作者：
S. Watanabe;T. Kaneko;K. Aida