A novel endoplasmic reticulum membrane protein Rcr1 regulates chitin deposition in the cell wall of Saccharomyces cerevisiae
A novel endoplasmic reticulum membrane protein Rcr1 regulates chitin deposition in the cell wall of Saccharomyces cerevisiae
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DOI：
10.1074/jbc.m409428200
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发表时间：
2005-03-04
影响因子：
4.8
通讯作者：
Yoda, K
中科院分区：
文献类型：
--
作者：
Imai, K;Noda, Y;Yoda, K
刚果红结合在细胞壁上，抑制酵母的生长。在对erd1Delta突变体的刚果红过敏性多拷贝抑制基因的筛选中，我们发现一个以前未描述的基因YBR005w使大多数酿酒酵母菌株对刚果红产生抗性。该基因被命名为RCR1(抗刚果红1号)。Rcr1Delta缺失突变体对刚果红的敏感度增加。RCR1编码一种具有单一跨膜区的新型内质网膜蛋白。分子解剖表明，跨膜区和部分C末端多肽具有足够的活性。我们检测了RCR1在与细胞壁相关的基因的各种零突变中的作用。突变体对刚果红的抗性与甲壳素含量的减少有关。多拷贝RCR1导致甲壳素含量显着下降，而碱溶葡聚糖的含量没有变化。在这些细胞中，硫代荧光白与细胞壁的结合显著减少。我们的结果表明，RCR1调节甲壳素的沉积，并提供了确凿的遗传和生化证据，表明刚果红的主要靶标是酿酒酵母中的甲壳素。
Congo red binds to the cell wall and inhibits the growth of yeast. In a screening for multicopy suppressor genes of Congo red hypersensitivity of erd1Delta mutant, we found that a previously uncharacterized gene, YBR005w, makes most of the Saccharomyces cerevisiae strains resistant to Congo red. This gene was named RCR1 ( resistance to Congo red 1). An rcr1Delta null mutant showed an increased sensitivity to Congo red. RCR1 encodes a novel ER membrane protein with a single transmembrane domain. Molecular dissection suggested that the transmembrane domain and a part of the C-terminal polypeptide are sufficient for the activity. We examined the effect of RCR1 in various null mutants of genes related to the cell wall. The resistance of mutants to Congo red correlates with a reduction of chitin content. Multicopy RCR1 caused a significant decrease in the chitin content while the amount of alkali-soluble glucan did not change. The binding of Calcofluor white to the cell wall significantly decreased in these cells. Our results show that RCR1 regulates the chitin deposition and add firm genetic and biochemical evidences that the primary target of Congo red is chitin in S. cerevisiae.