Screening cytokine/chemokine profiles in serum and organs from an endotoxic shock mouse model by LiquiChip
Screening cytokine/chemokine profiles in serum and organs from an endotoxic shock mouse model by LiquiChip
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通过 LiquiChip 筛选内毒素休克小鼠模型血清和器官中的细胞因子/趋化因子谱
DOI：
10.1007/s11427-016-9016-6
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发表时间：
2017-11-01
影响因子：
9.1
通讯作者：
Jiang, Yong
中科院分区：
文献类型：
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作者：
Liu, Jinghua;Wang, Juan;Jiang, Yong
研究脓毒症动物模型或患者的细胞因子谱，为确定诊断生物标志物和治疗靶点提供了实验依据。在本研究中，我们采用液相蛋白芯片（LiquiChip，也称为灵活多分析物分析技术）对多种细胞因子和趋化因子进行定量分析，这些因子包括白细胞介素-1β（IL-1β）、白细胞介素-2（IL-2）、白细胞介素-4（IL-4）、白细胞介素-5（IL-5）、白细胞介素-6（IL-6）、白细胞介素-10（IL-10）、白细胞介素-12（IL-12）、肿瘤坏死因子-α（TNF-α）、干扰素-γ（IFN-γ）、粒细胞 - 巨噬细胞集落刺激因子、角质细胞趋化因子、单核细胞趋化蛋白、γ干扰素诱导的单核因子、干扰素-γ诱导蛋白10以及巨噬细胞炎性蛋白1α。我们测定了脂多糖（LPS）攻击小鼠的血液以及包括肺、肝、心、肾、脾、脑、胃、肠和肌肉在内的多种组织中的这些细胞因子和趋化因子水平。我们的数据显示，LPS诱导的细胞因子在小鼠不同器官中的产生水平存在差异，且血液中的细胞因子水平与各器官中的并无相关性。我们还发现，大多数细胞因子水平在1至6小时内达到峰值，随后迅速下降。多种炎性细胞因子可能与脓毒性休克期间不同器官的损伤有关。我们的数据还表明，脾脏可能是全身炎症反应综合征和脓毒症发展过程中的一个重要靶器官。
Studying the cytokine profiles in animal models or patients with sepsis provides an experimental basis for the identification of diagnostic biomarkers and therapeutic targets. In this study, we used a liquid protein chip (LiquiChip), also known as flexible multi-analyte profiling technology, to perform quantitative analyses of several cytokines and chemokines (e.g., IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12, TNF-α, IFN-γ, granulocyte-macrophage colony-stimulating factor, keratinocyte chemoattractant, monocyte chemoattractant protein, monokine induced by gamma interferon, IFN-γ-inducible protein 10, and macrophage inflammatory protein 1 alpha). The levels of these cytokines and chemokines were determined both in the blood and in tissues, including the lung, liver, heart, kidney, spleen, brain, stomach, intestine and muscle, of mice challenged with LPS. Our data showed variable production levels of LPS-induced cytokines in different mouse organs, and the cytokine in the blood did not correlate with those in the organs. We also showed that the levels of most cytokines peaked within 1 to 6 h and decreased rapidly afterward. A variety of inflammatory cytokines might be related to the damage in different organs during septic shock. Our data also suggest that the spleen might be an important target organ in the development of systemic inflammatory response syndrome and sepsis.