Development of microsatellite markers in the gray ratsnake Ptyas korros (Colubridae)
Development of microsatellite markers in the gray ratsnake Ptyas korros (Colubridae)
复制标题
灰鼠蛇 Ptyas korros（游蛇科）微卫星标记的开发
DOI：
10.1007/s12686-013-0121-4
复制
发表时间：
2014-01
影响因子：
1.1
通讯作者：
Lin CX
中科院分区：
文献类型：
--
作者：
Zheng XX;Du Y;Lin LH;Lin CX
蛇是一种卵生杂交蛇，分布于中国东南部（包括台湾、海南和香港）、中南半岛、印度和印度尼西亚。根据《中国濒危动物红皮书》，这种蛇被列为易危物种。为了制定有效的保护策略，我们需要利用线粒体DNA和微卫星位点等分子标记来更好地了解该物种的基因流、种群结构和进化史。在这里，我们对10个多态微卫星位点进行了分析。从广西泉州种群中采集了57只。这些标记具有较高的遗传多样性（每个位点有4 ~ 14个等位基因）和杂合性（HO范围为0.296 ~ 1.000，HE范围为0.419 ~ 0.868）。没有基因座表现出明显偏离Hardy-Weinberg平衡。位点对间不存在连锁不平衡现象。这些微卫星标记将有助于研究柠条的基因流动、种群结构和进化史。
Ptyas korros is an oviparous colubrid snake that has a distribution covering the southeastern part of China (including Taiwan, Hainan and Hongkong), Indo-China Peninsula, India and Indonesia. The snake is listed as a vulnerable species according to the China Red Data Book of Endangered Animals. To develop effective conservation strategies for the snake, we need a better understanding of gene flow, population structure and evolutionary history of this species by using kinds of molecular markers such as mitochondrial DNA and microsatellite loci. Here, we characterize ten polymorphic microsatellite loci isolated from P. korros genomic libraries. Fifty-seven individuals were collected from Quanzhou population in Guangxi, China. These markers revealed a high degree of genetic diversity (4–14 alleles per locus) and heterozygosity (HO ranged from 0.296 to 1.000, and HE ranged from 0.419 to 0.868). No locus exhibited significant deviations from Hardy–Weinberg equilibrium. There was no evidence of linkage disequilibrium among pairs of loci. These microsatellite markers will be useful for the study of gene flow, population structure and evolutionary history of P. korros.
三个不同地理种群的灰鼠蛇 Ptyas korros 的繁殖特性
影响因子：
2.2
作者：
Lin, Long-Hui;Mao, Fei;Ji, Xiang
通讯作者：
Ji, Xiang
DOI：
--
发表时间：
1998
期刊：
--
影响因子：
--
作者：
Wang Sung;Zhao Ermi
通讯作者：
Wang Sung;Zhao Ermi