MDM2 Copy Numbers in Well-Differentiated and Dedifferentiated Liposarcoma
MDM2 Copy Numbers in Well-Differentiated and Dedifferentiated Liposarcoma
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DOI：
10.1309/ajcplyu89xhsnhqo
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发表时间：
2014-03-01
影响因子：
3.5
通讯作者：
Qasem, Shadi A.
中科院分区：
文献类型：
--
作者：
Ware, Patrick L.;Snow, Anthony N.;Qasem, Shadi A.
目的：MDM2基因扩增与高分化型和去分化型脂肪肉瘤相关。方法：对46例不同部位的脂肪肉瘤进行检测，其中22例为WDL，14例为DLL，10例为阴性对照。结果：WDL的MDM2扩增比(MDM2/CEP12)(10.2)明显低于DDL(18.3)(P=.0000002)。扩增比为16显示最佳的敏感性(0.86)和特异性(0.96)作为进展为DDL的分界点。交界区，定义为细胞增多和异型性增加但脂肪瘤分化保留的肿瘤，与WDL相比，MDM2比率显著升高(17.5；P=.0007)。中央型(腹膜后和腹内)肿瘤的MDM2比率也显著高于周围肿瘤(P=0.02)。结论：脂肪肉瘤MDM2扩增谱的差异有助于进一步定义和预测进展为高级别肿瘤。
Objectives: MDM2 gene amplification is associated with well-differentiated (WDL) and dedifferentiated liposarcomas (DDL). Using fluorescent in situ hybridization (FISH), we sought to characterize various patterns of MDM2 amplification among the morphologic spectrum of liposarcoma.Methods: Forty-six cases of liposarcoma in various sites were examined and included 22 WDLs, 14 DLLs, and 10 negative control subjects.Results: The MDM2 amplification ratio (MDM2/CEP12) was lower in WDL (10.2) compared with DDL (18.3) cases (P = .0000002). An amplification ratio of 16 showed optimal sensitivity (0.86) and specificity (0.96) as a cutoff point for progression to DDL. Borderline areas, defined as tumors with increased cellularity and atypia but with preserved lipomatous differentiation, showed a significantly higher MDM2 ratio (17.5; P = .0007) compared with WDL. Central (retroperitoneal and intra-abdominal) tumors also showed a significantly higher MDM2 ratio than peripheral ones (P = .02).Conclusions: Differences in MDM2 amplification profiles among liposarcomas could help further define and predict progression to high-grade neoplasia.