Hemoglobin albumin clusters as an artificial O2 carrier: Physicochemical properties and resuscitation from hemorrhagic shock in rats
Hemoglobin albumin clusters as an artificial O2 carrier: Physicochemical properties and resuscitation from hemorrhagic shock in rats
复制标题
血红蛋白白蛋白簇作为人工 O2 载体：理化特性和大鼠失血性休克的复苏
DOI：
10.1002/jbm.b.35040
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Teruyuki Komatsu
中科院分区：
文献类型：
--
作者：
Wataru Okamoto;Mai Hasegawa;Tomone Usui;Tomonori Kashima;Sho Sakata;Tatsuhiko Hamano;Hiroto Onozawa;Ryo Hashimoto;Masayuki Iwazaki;Mitsutomo Kohno;Teruyuki Komatsu
由人血清白蛋白 (HSA) 共价包裹的牛血红蛋白 (HbBv) 或成人血红蛋白 (HbA)、血红蛋白-白蛋白簇（HbBv-HSA3 和 HbA-HSA3）是用作红细胞替代品的人造 O2 载体。本文描述了 HbBv-HSA3 和 HbA-HSA3 溶液的理化特性，以及它们在麻醉大鼠失血性休克复苏后恢复全身状况的能力。 HbBv–HSA3 和 HbA–HSA3 具有高胶体渗透活性，表现出相同的溶液特性和 O2 结合参数。抽血50%诱发休克。大鼠表现出低血压和明显的代谢性酸中毒。 15 分钟后，给大鼠注射自体血 (SAB)、HbBv–HSA3、HbA–HSA3 或林格氏乳酸盐 (RL) 溶液。在失血性休克期间和给药后 6 小时内监测存活率、循环参数、血液学参数和血气参数。 SAB、HbBv-HSA3 和 HbA-HSA3 组的所有大鼠均存活 6 小时。 HbBv-HSA3和HbA-HSA3组在复苏后平均动脉压恢复。除RL组外，所有复苏组的血气参数时程均未观察到显着差异。血清生化检测显示，与 SAB 组相比，HbBv-HSA3 和 HbA-HSA3 组的天冬氨酸转氨酶 (AST) 和丙氨酸转氨酶 (ALT) 有所升高。因此，我们观察到其他大鼠在用 HbA-HSA3 复苏 7 天后苏醒。血细胞计数、AST 和 ALT 在 7 天时恢复到基线值。所有结果都表明，HbBv-HSA3 和 HbA-HSA3 簇可以作为 SAB 输血的替代材料提供失血性休克的恢复。
A bovine hemoglobin (HbBv) or human adult hemoglobin (HbA) wrapped covalently by human serum albumins (HSAs), hemoglobin–albumin clusters (HbBv–HSA3and HbA–HSA3), are artificial O2carriers used as a red blood cell substitute. This article describes the physicochemical properties of the HbBv–HSA3and HbA–HSA3solutions, and their abilities to restore the systemic condition after resuscitation from hemorrhagic shock in anesthetized rats. The HbBv–HSA3and HbA–HSA3, which have high colloid osmotic activity, showed equivalent solution characteristics and O2binding parameters. Shock was induced by 50% blood withdrawal. Rats exhibited hypotension and significant metabolic acidosis. After 15 min, the rats were administered shed autologous blood (SAB), HbBv–HSA3, HbA–HSA3, or Ringer's lactate (RL) solution. Survival rates, circulation parameters, hematological parameters, and blood gas parameters were monitored during the hemorrhagic shock and for 6 h after administration. All rats in the SAB, HbBv–HSA3, and HbA–HSA3groups survived for 6 h. The HbBv–HSA3and HbA–HSA3groups restored mean arterial pressure after the resuscitation. No remarkable difference was observed in the time courses of blood gas parameters in any resuscitated group except for the RL group. Serum biochemical tests showed increases in aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in the HbBv–HSA3and HbA–HSA3groups compared to the SAB group. Therefore, we observed other rats awakened after resuscitation with HbA–HSA3for 7 days. The blood cell count, AST, and ALT recovered to the baseline values by 7 days. All the results implied that HbBv–HSA3and HbA–HSA3clusters provide restoration from hemorrhagic shock as an alternative material for SAB transfusion.