Nonlinear optical microscopy and computational analysis of intrinsic signatures in breast cancer.
Nonlinear optical microscopy and computational analysis of intrinsic signatures in breast cancer.
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DOI：
10.1109/iembs.2009.5334523
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Eliceiri KW
中科院分区：
文献类型：
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作者：
Rueden CT;Conklin MW;Provenzano PP;Keely PJ;Eliceiri KW
最近，新的非侵入性成像方法已被开发并应用于细胞和动物乳腺模型，使乳腺癌研究人员能够跟踪乳腺转移的关键参与者和事件。非侵入性非线性光学方法，如多光子激光扫描显微镜（MPLSM），荧光寿命显微镜（FLIM）和二次谐波发生（SHG）成像提供了无与伦比的能力，从组织深处获得高分辨率图像，可用于了解乳腺癌的进展。这些光学方法可以通过允许对关键过程进行非侵入性成像来极大地增加我们对癌症进展的了解，例如代谢（基于通过FLIM的游离和结合的NADH检测）和与细胞外基质的相互作用（胶原蛋白的SHG成像）。在这篇简短的应用笔记中，我们介绍了我们最新的光学和计算工作的调查，以研究乳腺癌模型中的内源性荧光。特别是，我们目前在我们的SLIM绘图仪应用程序，一个开源的可视化程序的交互式可视化和检查组合光谱寿命（SLIM）数据的最新发展。
Recently, new non-invasive imaging methods have been developed and applied to cellular and animal mammary models that have enabled breast cancer researchers to track key players and events in mammary metastasis. Noninvasive nonlinear optical methods such as multiphoton laser scanning microscopy (MPLSM), Fluorescence Lifetime Microscopy (FLIM) and second harmonic generation (SHG) imaging provide an unrivaled ability for obtaining high-resolution images from deep within tissue that can be exploited in the quest to understand breast cancer progression. These optical methods can add greatly to our knowledge of cancer progression by allowing key processes to be non-invasively imaged such as metabolism (on the basis of free and bound NADH detection via FLIM) and interactions with the extracellular matrix (SHG imaging of collagen). In this short application note we present a survey of our latest optical and computational efforts to study intrinsic fluorescence in breast cancer models. In particular we present the latest development in our SLIM Plotter application, an open source visualization program for interactive visualization and inspection of combined spectral lifetime (SLIM) data.