Temporal transcription of two Antennapedia class homeobox genes in the marine copepod Calanus helgolandicus
Temporal transcription of two Antennapedia class homeobox genes in the marine copepod Calanus helgolandicus
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DOI：
10.1007/s10126-002-0119-5
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发表时间：
2003-11-01
影响因子：
3
通讯作者：
Smerdon, GR
中科院分区：
文献类型：
--
作者：
Lindeque, PK;Smerdon, GR
已经获得了在桡足动物发育中起关键作用的两个基因的DNA序列信息。第一个基因（Cal-antp）是通过筛选海芋（Calanus helgolandicus）基因组文库获得的。与蜈蚣（76.8%）和卤虾（75.5%）等生物的触角虫同源盒基因具有同源性。通过逆转录聚合酶链式反应（PCR）对从Calanus卵构建的固相cDNA文库进行反转录聚合酶链反应（PCR），随后使用基因组DNA模板进行反向PCR，获得第二个序列Hox 12。Hex - 12序列与Antennapedia类基因同源，与鸡HoxB3基因同源（71%，超过187 bp），与果蝇Sex Combs Reduced （Scr）同源基因同源（66%，超过223 bp）。利用Cal-Antp和hox12的序列数据，设计了特异性引物，通过PCR分析10个阶段特异性cDNA文库来研究这些基因的时间表达。
DNA sequence information has been obtained for 2 genes implicated in playing critical roles in copepod development. The first (Cal-antp) was obtained by screening a Calanus helgolandicus genomic library. It shows homology to Antennapedia homeobox genes from a number of organisms including the centipede (76.8% over 250 bp) and the brine shrimp (75.5% over 220 bp). A second sequence (Hox 12) was obtained through reverse transcription polymerase chain reaction (PCR) of a solid-phase cDNA library constructed from Calanus eggs, and subsequent inverse PCR using a genomic DNA template. The Hex 12 sequence is homologous to the Antennapedia class of genes and shares homology with the chicken HoxB3 gene (71% over 187 bp) and with the fruit fly Sex Combs Reduced (Scr) homeotic gene (66% identity over 223 bp). Using sequence data from Cal-Antp and Hox 12, specific primers were designed to investigate the temporal expression of these genes by PCR analysis of 10 stage-specific cDNA libraries.