Global Dynamics and Exchange Kinetics of a Protein on the Surface of Nanoparticles Revealed by Relaxation-Based Solution NMR Spectroscopy.
Global Dynamics and Exchange Kinetics of a Protein on the Surface of Nanoparticles Revealed by Relaxation-Based Solution NMR Spectroscopy.
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DOI：
10.1021/jacs.6b02654
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发表时间：
2016-05-11
影响因子：
15
通讯作者：
Clore GM
中科院分区：
文献类型：
--
作者：
Ceccon A;Tugarinov V;Bax A;Clore GM
的全球运动和交换动力学的模型蛋白质，泛素，结合到带负电荷的基于脂质的纳米颗粒（脂质体）的表面来自组合分析的交换寿命变宽所产生的结合到不同大小的纳米颗粒。滞留时间和旋转翻滚的结合蛋白质的总有效相关时间的相对贡献调制的纳米颗粒的大小，从而允许各种运动和交换参数被确定。泛素结合在大、小单层脂质体表面的停留时间均为20 μs。结合的泛素在低微秒时间尺度（约1.2 μs）上围绕近似垂直于脂质表面的轴进行内部旋转，同时在纳秒时间尺度上围绕内部旋转轴以30-55°为中心的圆锥体中摆动。泛素与脂质体的结合界面映射的分子间顺磁弛豫增强使用Gd 3+标记的囊泡，主要是带正电荷的表面正交的内部旋转轴。
The global motions and exchange kinetics of a model protein, ubiquitin, bound to the surface of negatively charged lipid-based nanoparticles (liposomes) are derived from combined analysis of exchange lifetime broadening arising from binding to nanoparticles of differing size. The relative contributions of residence time and rotational tumbling to the total effective correlation time of the bound protein are modulated by nanoparticle size, thereby permitting the various motional and exchange parameters to be determined. The residence time of ubiquitin bound to the surface of both large and small unilamellar liposomes is ∼20 μs. Bound ubiquitin undergoes internal rotation about an axis approximately perpendicular to the lipid surface on a low microsecond time scale (∼2 μs), while simultaneously wobbling in a cone of semiangle 30–55° centered about the internal rotation axis on the nanosecond time scale. The binding interface of ubiquitin with liposomes is mapped by intermolecular paramagnetic relaxation enhancement using Gd3+-tagged vesicles, to a predominantly positively charged surface orthogonal to the internal rotation axis.
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影响因子：
6.1
作者：
Anthis NJ;Clore GM
通讯作者：
Clore GM
DOI：
10.1615/critrevtherdrugcarriersyst.v26.i6.10
发表时间：
2009
影响因子：
2.7
作者：
Puri A;Loomis K;Smith B;Lee JH;Yavlovich A;Heldman E;Blumenthal R
通讯作者：
Blumenthal R
影响因子：
3.3
作者：
Wang, Ailin;Vo, Tam;Fitzkee, Nicholas C.
通讯作者：
Fitzkee, Nicholas C.
影响因子：
15
作者：
Zanzoni, Serena;Pedroni, Marco;Assfalg, Michael
通讯作者：
Assfalg, Michael
影响因子：
6.7
作者：
Zanzoni S;Ceccon A;Assfalg M;Singh RK;Fushman D;D'Onofrio M
通讯作者：
D'Onofrio M