CLP1, a Novel Plant Homeo Domain Protein, Participates in Regulating Cellulase Gene Expression in the Filamentous Fungus Trichoderma reesei
CLP1, a Novel Plant Homeo Domain Protein, Participates in Regulating Cellulase Gene Expression in the Filamentous Fungus Trichoderma reesei
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CLP1 是一种新型植物同源结构域蛋白，参与调节丝状真菌里氏木霉中的纤维素酶基因表达
DOI：
10.3389/fmicb.2019.01700
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发表时间：
2019-08-06
影响因子：
5.2
通讯作者：
Liu, Weifeng
中科院分区：
文献类型：
--
作者：
Wang, Lei;Yang, Renfei;Liu, Weifeng
丝状真菌里氏木霉中纤维素酶基因表达的严格调控网络涉及多个转录调控因子。然而，对这些监管者的识别和机制研究仍然不够。在这里，我们鉴定了一种新型转录调节因子 CLP1，它是一种参与调节里氏木霉纤维素酶基因表达的植物同源结构域 (PHD) 蛋白。系统发育分析表明，CLP1 同源物广泛分布于丝状真菌中，包括木霉属、青霉属、镰刀菌属、脉孢菌属和曲霉属物种。我们证明CLP1是一种核蛋白，CLP1的缺乏会显着损害纤维素酶基因的诱导表达。 ChIP 实验表明，在纤维素条件下，CLP1 与纤维素酶基因启动子特异性结合，并且在早期诱导阶段缺乏 CLP1 的情况下，会损害 XYR1 在相同启动子上的占据。 XYR1 过表达完全挽救了 clp1 无效突变体中纤维素酶生产的缺陷，但不能挽救分生孢子形成的缺陷。进一步分析表明，PHD 是 CLP1 适当亚细胞定位以及诱导纤维素酶基因表达和分生孢子形成所必需的。总之，这些数据证明了 CLP1 在里氏木霉中纤维素酶和木聚糖酶基因表达的调节中的重要作用。
The stringent regulatory network of cellulase gene expression in the filamentous fungus Trichoderma reesei involves multiple transcriptional regulators. However, identification and mechanistic investigation of these regulators are still insufficient. Here, we identified a novel transcriptional regulator, CLP1, a plant homeo domain (PHD) Protein that participates in regulating T. reesei cellulase gene expression. Phylogenetic analyses demonstrated that CLP1 homologs are widely distributed in filamentous fungi including Trichoderma, Penicillium, Fusarium, Neurospora, and Aspergillus species. We demonstrated that CLP1 is a nuclear protein and lack of CLP1 significantly impaired the induced expression of cellulase genes. ChIP experiments showed CLP1 binding to the cellulase gene promoters specifically under cellulose conditions and compromised XYR1 occupancy on the same promoters in the absence of CLP1 at the early induction stage. XYR1 overexpression fully rescued the defect in cellulase production but not the defect in conidia formation in the clp1 null mutant. Further analysis showed that the PHD is required for the CLP1 appropriate subcellular localization as well as the induced cellulase gene expression and conidiation. Taken together, these data demonstrated an important role of CLP1 in the regulation of cellulase and xylanase gene expression in T. reesei.