Combination of ischemic preconditioning and postconditioning can minimise skin flap loss: experimental study
Combination of ischemic preconditioning and postconditioning can minimise skin flap loss: experimental study
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DOI：
10.3109/2000656x.2016.1154468
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发表时间：
2016-01-01
影响因子：
1.2
通讯作者：
Tatlidede, Soner
中科院分区：
文献类型：
--
作者：
Akcal, Arzu;Sirvan, Selami Serhat;Tatlidede, Soner
目的：缺血预适应和后适应，是由一个或一系列短暂的缺血事件组成。本研究的目的是确定效率的后调节皮瓣在最小化皮瓣损失后预处理skin flaps.Methods：大鼠分为5组：假手术组，对照组，前CON组，后CON组，和前+后CON组。术后第3天和第7天，将整个皮瓣沿着坏死边缘描记在透明片上。结果：计算每只动物皮瓣坏死面积和坏死百分比。在第3天和第7天，发现对照组的坏死面积百分比显著高于其他组（分别为p=0.01和p=0.03）。在第7天，预处理组的坏死面积百分比显著高于预处理+处理后组（p=0.01）。VEGFR-3的表达率超过50%。通过增殖血管中VEGFR-3的免疫组织化学染色分别研究了晚期保护作用的存在。缺血预处理组、缺血后处理组、缺血前+缺血后处理组的皮瓣坏死面积均较缺血预处理组明显减少，缺血预处理+缺血后处理组的皮瓣坏死面积较缺血预处理组明显减少。通过增殖血管中VEGFR-3的免疫组织化学染色分别研究了晚期保护作用的存在。
Objectives: Ischaemic preconditioning and postconditioning, which consist of one or a series of short ischaemic events. This study aimed to determine the efficiency of post-conditioning a flap in the minimisation of flap loss after a preconditioned skin flap.Methods: The rats were divided into five groups: sham group, control group, pre-con group, post-con group, and pre+post-con group. On postoperative days 3 and 7, the entire flaps along with the margins of necrosis were traced onto transparent sheets. The areas of intact skin and tissue were recorded.Results: The flap necrosis area and percentage of necrosis were calculated for each animal. The necrotic area percentage of the control group was found to be significantly higher than those of the other groups on Days 3 and 7 (p=0.01 and p=0.03, respectively). The necrotic area percentage of the pre-con group was significantly higher than the pre+post-con group on Day 7 (p=0.01). VEGFR-3 expression was observed at a rate of more than 50% in the post-con group. The presence of a protective effect in the late period was separately investigated by immunohistochemical staining of VEGFR-3 in the proliferating vessels. The necrotic areas was reduced in the flaps of the pre-con, post-con, and pre+post-con groups and the combined preconditioning and postconditioning group has reduced necrotic area compared to preconditioning of the skin flap.Conclusion: The protective effect was observed on day 7 for combined ischaemic preconditioning and postconditioning. The presence of a protective effect in the late period was separately investigated by immunohistochemical staining of VEGFR-3 in the proliferating vessels.