Light scatter characteristics of blast cells in acute myeloid leukaemia: association with morphology and immunophenotype.
Light scatter characteristics of blast cells in acute myeloid leukaemia: association with morphology and immunophenotype.
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急性髓系白血病母细胞的光散射特征：与形态和免疫表型的关联。
DOI：
10.1136/jcp.48.5.456
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发表时间：
1995
影响因子：
3.4
通讯作者：
J. S. San Miguel
中科院分区：
文献类型：
--
作者：
M. Vidriales;A. Órfão;M. López;M. González;A. López;M. García;J. Galende;J. S. San Miguel
目的分析急性髓细胞白血病（AML）母细胞的前向散射/侧向散射（FSC/SSC）分布，以评估其是否与其形态学、免疫表型以及临床和生物学疾病特征相关。方法-FSC/SSC模式建立后，考虑到在FSC/SSC点图作为内部生物标准的残留T淋巴细胞的定位。分析了171例初治AML患者，建立了5种不同的FSC/SSC模式。考虑到骨髓抽吸物中正常细胞的FSC/SSC分布，这五种模式可以分为两大类：未成熟模式（1和2）和成熟模式（3、4和5）。这些FSC/SSC模式与AML患者的不同临床和生物学特征相关。结果-没有显着的协会被检测到有关的临床和血液疾病的特点和预后这些患者。相反，AML母细胞的FSC/SSC模式与FAB分类之间存在显著相关性。在显示成熟FSC/SSC模式的病例中（3、4和5），在整个系列中，甚至在每种FAB AML亚型中，检测到与髓样分化相关的抗原（如CD 13、CD 33、CD 11b、CD 15、CD 14、CD 4、CD 56和/或CD 16）的反应性增加（3、4和5）。相比之下，在显示不成熟FSC/SSC模式的那些病例中，对CD 34前体细胞相关抗原的反应性更高，甚至在每个FAB亚组中也观察到了这一点。结论-AML原始细胞的FSC/SSC模式分布不仅为这些白血病的分类提供了一个额外的客观和可重复的系统，而且它也可能代表FAB形态组和AML患者的免疫表型分类之间的联系。
AIMS--To analyse the forward scatter/side scatter (FSC/SSC) distribution of acute myeloblastic leukaemia (AML) blast cells in order to assess whether it correlates with their morphology, immunophenotype, and clinical and biological disease characteristics. METHODS--FSC/SSC patterns were established upon taking into account the localisation of the residual T lymphocytes in the FSC/SSC dot plot as an internal biological standard. One hundred and seventy one newly diagnosed AML patients were analysed and five different FSC/SSC patterns were established. These five patterns could be grouped into two major categories taking into account the FSC/SSC distribution of normal cells in a bone marrow aspirate: immature patterns (1 and 2) and mature patterns (3, 4, and 5). These FSC/SSC patterns were correlated with different clinical and biological characteristics of AML patients. RESULTS--No significant associations were detected in relation to the clinical and haematological disease characteristics and the prognosis of these patients. By contrast there was a significant correlation between the FSC/SSC pattern of the AML blast cells and the FAB classification. An increased reactivity for the antigens associated with myeloid differentiation such as CD13, CD33, CD11b, CD15, CD14, CD4, CD56, and/or CD16 was detected among cases showing a mature FSC/SSC pattern (3, 4, and 5), both in the whole series and even within each of the FAB AML subtypes. By contrast, the reactivity for the CD34 precursor cell associated antigen was higher among those cases displaying an immature FSC/SSC pattern, this being observed even within each FAB subgroup. CONCLUSIONS--The FSC/SSC pattern distribution of AML blast cells not only provides an additional objective and reproductible system for the classification of these leukaemias but it may also represent a connection between the FAB morphological groups and the immunophenotypic classification of AML patients.