A synergistic promotion strategy remarkably accelerated electrochemiluminescence of SnO2 QDs for MicroRNA detection using 3D DNA walker amplification.
A synergistic promotion strategy remarkably accelerated electrochemiluminescence of SnO2 QDs for MicroRNA detection using 3D DNA walker amplification.
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DOI：
10.1016/j.bios.2020.112820
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发表时间：
2020-11
影响因子：
12.6
通讯作者：
Fang Yang;Fang Yang;Ting-ting Tu;Ni Liao;Y. Chai;R. Yuan;Ying Zhuo
中科院分区：
文献类型：
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作者：
Fang Yang;Fang Yang;Ting-ting Tu;Ni Liao;Y. Chai;R. Yuan;Ying Zhuo
开发低成本和有效的方法来增强发光体的电化学发光（ECL）强度是非常可取和具有挑战性的。在此，我们开发了一种协同促进策略的基础上，三种类型的共反应促进剂，以实现高效的SnO 2量子点（SnO 2 QDs）为基础的三元电化学发光系统。具体而言，合理地选择MnO 2纳米花（MnO 2 NFs），银纳米粒子（Ag NPs）和氯化血红素/G-四链体作为共反应加速剂。由于协同效应，三种共反应促进剂的巧妙整合使得结构稳定性更好，暴露的催化活性位点更多，电荷转移更快，从而比单一共反应促进剂更有效地促进共反应物（S2 O 82-）的还原。为了证明该原理的实用性，结合3D DNA步行者构建了“开-关-超级开”ECL生物传感器，其显示出用于高灵敏度miRNA-21检测的上级线性范围（10 aM-100 pM）和低检测限（2.9 aM）。总之，本工作首次报道了将三种共反应促进剂巧妙地结合在一起，显著增强SnO 2量子点的电致化学发光，为研究高效共反应促进剂的结构和组分的巧妙设计提供了全新的视角。
Developing low-cost and efficient methods to enhance the electrochemiluminescence (ECL) intensity of luminophores is highly desirable and challenging. Herein, we develop a synergistic promotion strategy based on three types of co-reaction accelerators to achieve an efficient SnO2quantum dots (SnO2QDs)-based ternary ECL system. Specifically, the MnO2nanoflowers (MnO2NFs), Ag nanoparticles (Ag NPs) and hemin/G-quadruplex were rationally selected as co-reaction accelerators. Owing to the synergistic effect, the deft integration of three types of co-reaction accelerators enabled better structural stability, more exposed catalytic active sites, and faster charge transfer, thus more effectively facilitating the reduction of co-reactant (S2O82−) compared with that of the single co-reaction accelerator. To demonstrate the practical utility of this principle, an “on-off-super on” ECL biosensor was constructed in combination with a 3D DNA walker, which showed a superior linear range (10 aM–100 pM) and a low detection limit (2.9 aM) for the highly-sensitive miRNA-21 detection. In general, this work firstly reported that three types of co-reaction accelerators were deftly integrated to remarkably amplify the ECL emission of SnO2QDs, and provided brand-new perspectives for research on the ingenious design of the structure and component of highly efficient co-reaction accelerators.